Ruocco L A, Viggiano D, Pignatelli M, Iannaccone T, Rimoli M G, Melisi D, Curcio A, De Lucia S, Carboni E, Gironi Carnevale U A, de Caprariis P, Sadile A G
Department of Experimental Medicine, II University of Naples, Italy.
Behav Brain Res. 2008 Mar 5;187(2):449-54. doi: 10.1016/j.bbr.2007.10.007. Epub 2007 Oct 13.
Different strategies can be used to carry dopamine into the brain such as L-Dopa precursors or galactosilated form of DA (GAL-DA). The aim of this study was to investigate whether GAL-DA would reduce hyperactivity and increase non-selective attention (NSA) in a mouse model of attention deficit hyperactivity disorder (ADHD), as, i.e. C57BL/6 as did in NHE rats. Here we report that GAL-DA increases NSA in a spatial novelty in C57BL/6 mice. They received a single i.p. injection of GAL-DA (10 mg/kg or 100 mg/kg) or equimolar galactose vehicle. Another mouse strain the Swiss albino was introduced as inbred control group. Three hours after last injection mice were tested in a Làt-maze for 30-min. Behaviour was analyzed for horizontal (traveled distance) and vertical activity (orienting frequency and scanning durations) which shares cognitive and non-cognitive nature, respectively. Ten milligram per kilograms of GAL-DA, increases scanning duration in C57BL/6 mice. Thus a low dose of GAL-DA increases NSA without reducing hyperactivity in this mouse model of ADHD.
可以采用不同策略将多巴胺输送到大脑，比如左旋多巴前体或多巴胺的半乳糖化形式（GAL-DA）。本研究的目的是探究GAL-DA是否会像在NHE大鼠中那样，在注意力缺陷多动障碍（ADHD）小鼠模型（如C57BL/6小鼠）中减少多动并增加非选择性注意力（NSA）。在此我们报告，GAL-DA增加了C57BL/6小鼠在空间新奇环境中的NSA。它们接受了一次腹腔注射GAL-DA（10毫克/千克或100毫克/千克）或等摩尔的半乳糖载体。引入了另一种小鼠品系瑞士白化小鼠作为近交对照组。在最后一次注射三小时后，小鼠在L形迷宫中接受30分钟测试。分别对具有认知和非认知性质的水平活动（行进距离）和垂直活动（定向频率和扫描持续时间）进行行为分析。每千克10毫克的GAL-DA增加了C57BL/6小鼠的扫描持续时间。因此，在这个ADHD小鼠模型中，低剂量的GAL-DA增加了NSA但没有减少多动。
