Shen H H, Liu F G, Hu Y M
Department of Medicine, Second Affiliated Hospital, Zhejiang Medical University, Hangzhou.
Zhonghua Nei Ke Za Zhi. 1991 Sep;30(9):539-41, 593.
Human basophil releasability (HBR) was determined in 50 patients with asthma by modified human basophil degranulation test (HBDT). The result of HBDT was expressed as degranulation index (DI). 1. Anti-IgE-induced HBR: the mean value of DI in extrinsic asthma, intrinsic asthma and control subjects was 50.1 +/- 2.26(%), 32.2 +/- 2.3(%) and 18 +/- 2.74(%) (x +/- sem) respectively. If a DI of 30% or greater was considered as positive, the positive rate in the above three groups would be 96.9%, 60.0% and 20.0% respectively. There was significant difference between any two of the three groups (P less than 0.01). 2. Allergen-induced HBR: HBDT was performed in the asthmatics with sixteen allergens and in the controls with mite allergen. The positive rates were 68% in the patients and 0% in the controls. The coincident rate of the results between HBDT and skin test reached 82.9%. The results showed that anti-IgE-induced HBR could be regarded as an objective parameter for differentiating extrinsic and intrinsic asthma and allergen-induced HBR could be considered as an useful means for in vitro allergen diagnosis of asthma.
采用改良的人嗜碱性粒细胞脱颗粒试验（HBDT）测定了50例哮喘患者的人嗜碱性粒细胞释放能力（HBR）。HBDT结果以脱颗粒指数（DI）表示。1. 抗IgE诱导的HBR：外源性哮喘、内源性哮喘和对照组的DI平均值分别为50.1±2.26（%）、32.2±2.3（%）和18±2.74（%）（x±标准误）。若将DI≥30%视为阳性，则上述三组的阳性率分别为96.9%、60.0%和20.0%。三组中任意两组之间均有显著差异（P＜0.01）。2. 变应原诱导的HBR：对哮喘患者用16种变应原进行HBDT，对对照组用螨变应原进行HBDT。患者组阳性率为68%，对照组为0%。HBDT与皮肤试验结果的符合率达82.9%。结果表明，抗IgE诱导的HBR可作为鉴别外源性和内源性哮喘的客观指标，变应原诱导的HBR可作为哮喘体外变应原诊断的有用手段。
