Wang Z
Dept. Urology, Zhongshan Hospital, Shanghai Medical University.
Zhonghua Zhong Liu Za Zhi. 1991 Jul;13(4):245-8.
The cellular DNA content was quantitatively and the expression of oncogene ras P 21 qualitatively assessed in paraffin embedded tumor specimens from 62 patients with bladder tumors, using the paraffin-embedded bladder mucosa specimens from 7 patients with non-neoplastic urinary diseases as control. The results were retrospectively analysed in relation to the outcome of patients on follow-up. In 25 cases with superficial bladder tumor the positive rate of P 21 expression and the frequency of aneuploid were 92% and 12%, respectively. But in 37 cases with infiltrative bladder tumor the positive rate of P 21 expression and the frequency of aneuploid were 29.7% and 78.3%, respectively. It was considered that the P 21 expression may imply early bladder tumor whereas the finding of aneuploid was a parameter of advanced bladder tumor. Our results also showed that the expression or non-expression of P 21 was closely related to DNA ploid patterns. The positive rate of P 21 expression was 90% in the diploid bladder tumors and 50% in the tetraploid bladder tumors. The P 21 expression was negative in all the cases with double aneuploid bladder tumors. The results suggest that DNA ploid patterns and P 21 expression, when analyzed simultaneously, would be prognostically more informative than either alone.
对62例膀胱肿瘤患者石蜡包埋肿瘤标本中的细胞DNA含量进行了定量分析，并对癌基因ras P 21的表达进行了定性评估，以7例非肿瘤性泌尿系统疾病患者的石蜡包埋膀胱黏膜标本作为对照。对结果进行回顾性分析，并与患者的随访结果相关联。在25例浅表性膀胱肿瘤中，P 21表达阳性率和非整倍体频率分别为92%和12%。但在37例浸润性膀胱肿瘤中，P 21表达阳性率和非整倍体频率分别为29.7%和78.3%。认为P 21表达可能意味着早期膀胱肿瘤，而非整倍体的发现是晚期膀胱肿瘤的一个参数。我们的结果还表明，P 21的表达或不表达与DNA倍体模式密切相关。二倍体膀胱肿瘤中P 21表达阳性率为90%，四倍体膀胱肿瘤中为50%。所有双非整倍体膀胱肿瘤病例中P 21表达均为阴性。结果表明，同时分析DNA倍体模式和P 21表达，在预后方面比单独分析任何一项更具信息价值。
