Ngure R M, Eckersall P D, Jennings F W, Mburu J, Burke J, Mungatana N, Murray M
Department of Veterinary Clinical Biochemistry, Faculty of Veterinary Medicine, University of Glasgow, Glasgow, UK.
Vet Parasitol. 2008 Jan 25;151(1):14-20. doi: 10.1016/j.vetpar.2007.10.007. Epub 2007 Oct 22.
Mice infected with Trypanosoma congolense developed a severe anaemia 1 week after infection, which persisted till treatment with diminazine aceturate when the packed cell volume (PCV) recovered to pre-infection levels. This was accompanied by a marked increase in the plasma levels of the acute phase proteins (APP), serum amyloid P-component (SAP) and haptoglobin (Hp). The initial peak levels of Hp and SAP were attained 7 and 12 days post-infection (DPI), respectively. Thereafter SAP levels decreased significantly to near pre-infection levels, but later increased even after treatment to give a second peak 34 DPI after which there was a decline till the study was terminated. The Hp levels on the other hand decreased to an intermediate level after the initial peak increasing to a second peak 22 DPI. Thereafter Hp decreased significantly following diminazine aceturate treatment to reach pre-infection levels within 5 days post-treatment. This indicates that T. congolense-infected mice develop severe anaemia accompanied by an acute phase response leading to an increase in SAP and Hp but that following treatment divergent responses occurred indicating differences in the pathways for stimulation of the APP. Haptoglobin was shown to be an earlier indicator of infection and a better marker in monitoring the response to treatment.
感染刚果锥虫的小鼠在感染后1周出现严重贫血，这种贫血一直持续到用乙酰氨基苯脒治疗时，红细胞压积（PCV）恢复到感染前水平。这伴随着急性期蛋白（APP）、血清淀粉样蛋白P成分（SAP）和触珠蛋白（Hp）血浆水平的显著升高。Hp和SAP的初始峰值水平分别在感染后7天和12天达到。此后，SAP水平显著下降至接近感染前水平，但后来即使在治疗后仍有所升高，在感染后34天出现第二个峰值，之后直至研究结束一直下降。另一方面，Hp水平在初始峰值后降至中间水平，在感染后22天升至第二个峰值。此后，在用乙酰氨基苯脒治疗后，Hp显著下降，在治疗后5天内达到感染前水平。这表明感染刚果锥虫的小鼠会出现严重贫血并伴有急性期反应，导致SAP和Hp升高，但治疗后出现了不同的反应，表明刺激APP的途径存在差异。触珠蛋白被证明是感染的早期指标，也是监测治疗反应的更好标志物。
