Kuwana T, Sugita O, Yakata M
Department of Laboratory Medicine, Niigata University School of Medicine, Japan.
Enzyme. 1991;45(1-2):63-6. doi: 10.1159/000468866.
Fractionation of bone and liver alkaline phosphatase (EC 3.1.3.1; ALP) in serum by serial lectin affinity chromatography has demonstrated differences in the sugar chain structure of bone and liver ALP in serum from that previously reported in the corresponding tissues, with a lower content of high mannose or hybrid-type sugar chains and a higher content of biantennary complex-type chains. Furthermore, the bone and liver ALPs were found to differ in the latter with the bone fraction showing a greater content of fucose residues.
通过连续凝集素亲和层析对血清中的骨碱性磷酸酶和肝碱性磷酸酶（EC 3.1.3.1；ALP）进行分级分离，结果表明血清中骨碱性磷酸酶和肝碱性磷酸酶的糖链结构与之前在相应组织中报道的有所不同，高甘露糖型或杂合型糖链含量较低，双触角复合型糖链含量较高。此外，还发现骨碱性磷酸酶和肝碱性磷酸酶在后者存在差异，骨部分的岩藻糖残基含量更高。
