Myrset A H, Helgeland L
Department of Biochemistry, University of Oslo, Norway.
J Immunoassay. 1991;12(4):597-609. doi: 10.1080/01971529108053282.
An enzyme linked immunoassay (ELISA) for quantitation of rat prothrombin, based on a biotin-streptavidin alkaline phosphatase system is described. The assay utilizes rabbit antiserum raised against purified rat prothrombin. The assay was twenty fold more sensitive than a rat prothrombin assay based on amidolytic activity following activation by Echis carinatus venom. Results obtained with the two assays show good correlation. The ELISA is a valuable tool for quantitation of minute amounts of prothrombin in subcellular fractions and large series of plasma samples.
本文描述了一种基于生物素-链霉亲和素碱性磷酸酶系统的用于定量大鼠凝血酶原的酶联免疫吸附测定（ELISA）。该测定使用针对纯化的大鼠凝血酶原产生的兔抗血清。该测定比基于锯鳞蝰蛇毒激活后的酰胺水解活性的大鼠凝血酶原测定法灵敏20倍。两种测定法得到的结果显示出良好的相关性。ELISA是定量亚细胞组分和大量血浆样品中微量凝血酶原的有价值工具。
