使用枯草芽孢杆菌基因组载体进行自下而上的基因组组装。
Bottom-up genome assembly using the Bacillus subtilis genome vector.
作者信息
Itaya Mitsuhiro, Fujita Kyoko, Kuroki Azusa, Tsuge Kenji
机构信息
Mitsubishi Kagaku Institute of Life Sciences, 11 Minamiooya, Machida-shi, Tokyo 194-8511, Japan.
出版信息
Nat Methods. 2008 Jan;5(1):41-3. doi: 10.1038/nmeth1143. Epub 2007 Dec 9.
We established a protocol to construct complete recombinant genomes from their small contiguous DNA pieces and obtained the genomes of mouse mitochondrion and rice chloroplast using a B. subtilis genome (BGM) vector. This method allows the design of any recombinant genomes, valuable not only for fundamental research in systems biology and synthetic biology but also for various applications in the life sciences.
我们建立了一种从小的连续DNA片段构建完整重组基因组的方案，并使用枯草芽孢杆菌基因组（BGM）载体获得了小鼠线粒体和水稻叶绿体的基因组。这种方法允许设计任何重组基因组，不仅对系统生物学和合成生物学的基础研究有价值，而且对生命科学中的各种应用也有价值。
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