Türk G, Gür S, Sönmez M, Bozkurt T, Aksu E H, Aksoy H
Department of Reproduction and Artificial Insemination, Faculty of Veterinary Medicine, Fırat University, Elazığ, Turkey.
Reprod Domest Anim. 2008 Jun;43(3):308-313. doi: 10.1111/j.1439-0531.2007.00896.x. Epub 2007 Dec 7.
This study was carried out to investigate the efficacy of PGF(2alpha) for oestrus synchronization (ES) in Awassi ewes to which were administered the progestagen-PMSG combination, and to evaluate the effect of the exogenous GnRH administration immediately after the artificial insemination (AI) on their pregnancy rate and lambing performance during the breeding season. The ewes (n = 33) were treated with an intravaginal sponge impregnated with 30 mg fluorogestane acetate for 12 days and were injected with 500 IU PMSG at the time of removal of the sponge. The ewes were then divided into three equal groups of 11 ewes each. One millilitre of physiological saline (0.9% NaCl; placebo) was administered to each ewe in Group 1 at the time of second AI. Approximately 4 microg GnRH (busereline) was injected to each ewe in Group 2 immediately after second AI. A total of 150 microg PGF(2alpha) (cloprostenole) was injected at the time of sponge removal on day 12 and 4 microg GnRH immediately after the second AI was also treated to each ewe in Group 3. Intracervical AI with diluted fresh semen was performed twice at 12 and 24 h following the onset of oestrus. The injection-oestrus onset and injection-oestrus-end interval in Group 3 was significantly (p < 0.001) shorter than both Groups 1 and 2. Although the pregnancy rates of Groups 2 and 3 (81.8%; 9/11) were numerically higher than of Group 1 (63.6%; 7/11), the difference among the groups was statistically insignificant. The multiple birth rate of Group 3 was found higher than Groups 1 and 2. However, the number of single lambs of Group 1 was also higher than Groups 2 and 3 (p < 0.05). Despite the litter sizes of Groups 2 (1.27; 14/11) and 3 (1.55; 17/11) being numerically higher than Group 1 (0.73; 8/11), the differences among all the groups were statistically insignificant. In conclusion, the administration of PGF(2alpha) at the time of removal of the sponge shortens the injection oestrus-onset and oestrus-end interval in Awassi ewes treated with progestagen-PMSG. Additionally, exogenous GnRH treatment immediately after the AI increases the multiple birth rate of Awassi ewes synchronized with progestagen-PMSG-PGF(2alpha) combination.
本研究旨在调查前列腺素F2α（PGF2α）对接受孕激素 - 孕马血清促性腺激素（PMSG）组合处理的阿瓦西母羊发情同步化（ES）的效果，并评估人工授精（AI）后立即给予外源性促性腺激素释放激素（GnRH）对其繁殖季节妊娠率和产羔性能的影响。33只母羊用含30mg醋酸氟孕酮的阴道海绵栓处理12天，并在取出海绵栓时注射500IU PMSG。然后将母羊分为三组，每组11只。在第二次AI时，给第1组的每只母羊注射1ml生理盐水（0.9%氯化钠；安慰剂）。在第二次AI后立即给第2组的每只母羊注射约4μg GnRH（布舍瑞林）。在第12天取出海绵栓时，给第3组的每只母羊总共注射150μg PGF2α（氯前列醇），并在第二次AI后立即注射4μg GnRH。在发情开始后的12小时和24小时，用稀释的新鲜精液进行两次子宫颈内AI。第3组的注射 - 发情开始和注射 - 发情结束间隔明显短于第1组和第2组（p < 0.001）。虽然第2组和第3组的妊娠率（81.8%；9/11）在数值上高于第1组（63.6%；7/11），但组间差异无统计学意义。发现第3组的多羔率高于第1组和第2组。然而，第1组的单羔数量也高于第2组和第3组（p < 0.05）。尽管第2组（1.27；14/11）和第3组（1.55；17/11）的产仔数在数值上高于第1组（0.73；8/11），但所有组之间的差异无统计学意义。总之，在取出海绵栓时给予PGF2α可缩短接受孕激素 - PMSG处理的阿瓦西母羊的注射发情开始和发情结束间隔。此外，AI后立即进行外源性GnRH处理可提高与孕激素 - PMSG - PGF2α组合同步发情的阿瓦西母羊的多羔率。
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