Department of Progettazione e Gestione dei Sistemi Agro-Zootecnici e Forestali - PRO.GE.S.A., University of Bari, Via Amendola 165/A, Bari, Italy.
Anim Reprod Sci. 2011 Jan;123(1-2):32-9. doi: 10.1016/j.anireprosci.2010.11.007. Epub 2010 Nov 25.
Two experiments were conducted in ewes in order to develop an oestrus-ovulation short time synchronization protocol based on combined FGA, PGF(2α), GnRH, eCG treatments, for use in dairy sheep before natural service (Experiment 1) or for fixed-time artificial insemination (Experiment 2), during the breeding season. In Experiment 1 seventy-five non-lactating dairy ewes were subdivided into 5 treatment groups (N=15): (1) Group Fe - control, which received FGA vaginal sponges (14 days)+eCG (Day 14); (2) Group FPe, FGA (5 days)+PGF(2α) (Day 5)+eCG (Day 5); (3) Group PFe, PGF(2α) (Day 0)+FGA (5 days)+eCG (Day 5); (4) Group PFG, PGF(2α) (Day 0)+FGA (5 days)+GnRH (30h after sponge removal, s.r.); (5) Group GPe, GnRH (Day 0)+PGF(2α) (Day 5)+eCG (Day 5). Ewes were checked for oestrus and hand-mated. Time of ovulation was recorded by laparoscopy for 10 animals from each treatment. The percentages of female in oestrus and the interval to oestrus (h after treatment), fertility and prolificacy rate were recorded. There were no treatment differences in the percentage of females in oestrus. The interval to oestrus was earlier in Fe Group and delayed in FPe Group (P<0.01). Ovulation time was earlier in GPe Group compared to FPe Group (P<0.05). Fertility rates were significantly different (P<0.05) between the PFe and the FPeG Groups compared with the PFG Group. No significant differences were observed in prolificacy among the treatments. In Experiment 2, sixty dry ewes were subdivided (N=20) into the following three experimental treatment groups: (1) Group FP, FGA (5 days)+PGF(2α) (Day 5); (2) Group FPG, FGA (5 days)+PGF(2α) (Day 5)+GnRH (30hs.r.); (3) Group FPeG, FGA (5 days)+PGF(2α) (Day 5)+eCG (Day 5)+GnRH (30hs.r.). These were further subdivided into two groups (N=10) corresponding to 52 and 60hs.r. fixed-time insemination. Laparoscopic intrauterine insemination was performed with frozen semen (80×10(6)spermatozoa/dose) and ovulation time was recorded in a subgroup (N=10). GnRH resulted in an earlier ovulation time (P<0.05) in FPG and FPeG Groups (53.0h vs 61.6h). Fertility rate was higher in FPeG treated ewes inseminated at 60hs.r. (60%, 6/10). In FP and FPG Groups fertility rates were higher following insemination at 52hs.r. (50.0 and 40.0%).
为了开发一种基于联合 FGA、PGF(2α)、 GnRH、eCG 处理的发情-排卵短时间同步方案，用于奶牛绵羊在自然配种前（实验 1）或定时人工授精（实验 2）期间，在繁殖季节进行了两项实验。在实验 1 中，75 只非泌乳奶牛母羊被分为 5 个处理组（N=15）：（1）组 Fe-对照，接受 FGA 阴道海绵（14 天）+eCG（第 14 天）；（2）组 FPe，FGA（5 天）+PGF(2α)（第 5 天）+eCG（第 5 天）；（3）组 PFe，PGF(2α)（第 0 天）+FGA（5 天）+eCG（第 5 天）；（4）组 PFG，PGF(2α)（第 0 天）+FGA（5 天）+GnRH（海绵去除后 30 小时，s.r.）；（5）组 GPe，GnRH（第 0 天）+PGF(2α)（第 5 天）+eCG（第 5 天）。检查母羊发情并进行人工授精。每组 10 只动物通过腹腔镜记录排卵时间。记录发情母羊的百分比和发情间隔（治疗后 h）、受精率和产仔率。发情母羊的百分比在处理组之间没有差异。发情间隔在 Fe 组较早，在 FPe 组较晚（P<0.01）。与 FPe 组相比，GPe 组的排卵时间较早（P<0.05）。与 PFG 组相比，PFe 和 FPeG 组的受精率有显著差异（P<0.05）。处理之间的产仔率没有显著差异。在实验 2 中，60 只干母羊被分为（N=20）以下三个实验处理组：（1）组 FP，FGA（5 天）+PGF(2α)（第 5 天）；（2）组 FPG，FGA（5 天）+PGF(2α)（第 5 天）+GnRH（30hs.r.）；（3）组 FPeG，FGA（5 天）+PGF(2α)（第 5 天）+eCG（第 5 天）+GnRH（30hs.r.）。这些进一步分为两组（N=10），对应于 52 和 60hs.r.定时授精。用冷冻精液（80×10(6)精子/剂量）进行腹腔镜宫内授精，并在亚组（N=10）中记录排卵时间。GnRH 使 FPG 和 FPeG 组的排卵时间更早（P<0.05）（53.0h 对 61.6h）。在 60hs.r.授精的 FPeG 处理母羊中，受精率更高（60%，6/10）。在 FP 和 FPG 组中，在 52hs.r.授精时，受精率更高（50.0%和 40.0%）。
