Cai Jian-Chun, Liu Di, Zhang Hai-ping, Zhong San, Xia Nig-shao
Department of Oncological Surgery, Xiamen Cancer Center, Xiamen First Hospital, Affiliated to Fujian Medical University, China.
Zhonghua Zhong Liu Za Zhi. 2007 Jul;29(7):510-3.
To study the promoter hypermethylation of several tumor suppressor genes in gastric carcinoma (GC) tissue and adjacent normal gastric foveolar epithelium (GFE).
Methylation specific PCR (MSP) was used to examine the promoter methylation of tumor suppressor genes E-cadherin, hMLH1, APC and MGMT in paraffin-embedded gastric cancer tissue and adjacent normal foveolar epithelium in 106 cases.
The positive rate of genes promoter methylation was 44.3% (47/106 cases) and 72.6% (77/106 cases) at one or more genes tested in the normal GFE and GC tissue, respectively. There was a significant difference in the positive rates of gene promoter methylation between normal GFE and GC tissue (P = 0.0001). There was a significant association with Laurén classification, degree of differentiation and pTNM staging in GC (P < 0.05), but no significant association with Ming's classification (P > 0.05).
Tumor suppressor genes promoter methylation is frequently present in GC and adjacent normal gastric foveolar epithelium, especially in Laurén diffuse type GC, poorly differentiated GC, mucus-secreting (signet ring) cell GC and pTNM stage III and IV GC. Our findings indicate that the gene promoter methylation is a common and early event in GC carcinogensis.
研究胃癌（GC）组织及癌旁正常胃小凹上皮（GFE）中几种抑癌基因的启动子高甲基化情况。
采用甲基化特异性PCR（MSP）检测106例石蜡包埋胃癌组织及癌旁正常小凹上皮中抑癌基因E-钙黏蛋白、hMLH1、APC和MGMT的启动子甲基化情况。
在正常GFE和GC组织中，一个或多个检测基因的启动子甲基化阳性率分别为44.3%（47/106例）和72.6%（77/106例）。正常GFE和GC组织间基因启动子甲基化阳性率有显著差异（P = 0.0001）。在GC中，启动子甲基化与Laurén分型、分化程度及pTNM分期有显著相关性（P < 0.05），但与Ming分型无显著相关性（P > 0.05）。
抑癌基因启动子甲基化在GC及癌旁正常胃小凹上皮中常见，尤其在Laurén弥漫型GC、低分化GC、黏液分泌（印戒）细胞GC及pTNM III期和IV期GC中。我们的研究结果表明基因启动子甲基化是GC发生过程中的常见早期事件。
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