MGMT和hMLH1基因的异常DNA甲基化在胃癌预测中的作用
Aberrant DNA methylation of MGMT and hMLH1 genes in prediction of gastric cancer.
作者信息
Jin J, Xie L, Xie C H, Zhou Y F
机构信息
Department of Radiation & Medical Oncology, Zhongnan Hospital of Wuhan University, Clinical Cancer Study Center of Hubei Province, Key Laboratory of Tumor Biological Behavior of Hubei Province, Wuhan, China.
Ezhou Central Hospital, Ezhou, Hubei, China.
出版信息
Genet Mol Res. 2014 May 30;13(2):4140-5. doi: 10.4238/2014.May.30.9.
We aimed to explore the association between aberrant DNA methylation of the O(6)-methylguanine-DNA methyltransferase (MGMT) and human mutL homolog 1 (hMLH1) genes with gastric cancer. A total of 283 gastric cancer patients who were confirmed by pathological diagnosis were included in our study. Aberrant DNA methylation of MGMT and hMLH1 were detected. The proportions of DNA hypermethylation in MGMT and hMLH1 in cancer tissues were significantly higher than those in remote normal-appearing tissues. The DNA hypermethylation of MGMT was correlated with the tumor-necrosis-metastasis stage in gastric cancer tissues. Results showed that individuals with gastric cancer in the N1 and M1 stages had a significantly higher risk of DNA hypermethylation of MGMT in cancer tissues [odds ratio (OR) = 1.97, 95% confidence interval (CI) = 1.15-3.37 for the N1 stage; OR (95%CI) = 5.39 (2.08-14.98) for the M1 stage]. In conclusion, we found that aberrant hypermethylation of MGMT could be a predictive biomarker for detecting gastric cancer.
我们旨在探讨O(6)-甲基鸟嘌呤-DNA甲基转移酶（MGMT）和人类错配修复蛋白1（hMLH1）基因的异常DNA甲基化与胃癌之间的关联。本研究纳入了283例经病理诊断确诊的胃癌患者。检测了MGMT和hMLH1的异常DNA甲基化情况。癌组织中MGMT和hMLH1的DNA高甲基化比例显著高于远隔的正常外观组织。胃癌组织中MGMT的DNA高甲基化与肿瘤坏死转移分期相关。结果显示，处于N1和M1期的胃癌患者癌组织中MGMT的DNA高甲基化风险显著更高 [N1期的比值比（OR）= 1.97，95%置信区间（CI）= 1.15 - 3.37；M1期的OR（95%CI）= 5.39（2.08 - 14.98）]。总之，我们发现MGMT的异常高甲基化可能是检测胃癌的一种预测性生物标志物。
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