Brichta-Harhay D M, Arthur T M, Koohmaraie M
US Department of Agriculture, Agricultural Research Service, Roman L. Hruska US Meat Animal Research Center, Clay Center, Nebraska 68933-0166, USA.
Lett Appl Microbiol. 2008 Feb;46(2):186-91. doi: 10.1111/j.1472-765X.2007.02289.x. Epub 2007 Dec 7.
To evaluate direct plating methods for the estimation of Salmonella load in poultry carcass rinses.
Two direct plating tools, the spiral plate count method (SPCM) and the hydrophobic grid membrane filtration (HGMF) method, were adapted to support quantification of Salmonella during poultry processing. Test samples consisted of 180 broiler carcasses from a commercial abattoir, 60 from each of three points in the processing line [pre-inside-outside bird wash (pre-IOBW), prechill and postchill]. The SPCM was used to estimate Salmonella load in pre-IOBW rinses, while HGMF was used to estimate Salmonella levels in prechill and postchill rinses. Carcass rinses were also evaluated for Salmonella prevalence by enrichment methods. Mean prevalences of Salmonella were 95%, 100% and 41.7%, and the geometric mean loads were 3.7 x 10(1), 5.6 x 10(0) and 5.0 x 10(-2) CFU ml(-1) for pre-IOBW, prechill and postchill rinses, respectively.
The methods described are useful for estimating the concentration of viable and typical Salmonella in poultry carcass rinses.
Direct plating enumeration methods can facilitate the monitoring of Salmonella load on poultry carcasses throughout the production process, and the evaluation of new processing intervention strategies.
评估直接平板计数法用于估计家禽胴体冲洗液中沙门氏菌载量的效果。
采用两种直接平板计数工具，即螺旋平板计数法（SPCM）和疏水网格膜过滤法（HGMF），以支持在家禽加工过程中对沙门氏菌进行定量分析。测试样品包括来自一家商业屠宰场的180只肉鸡胴体，在加工生产线的三个点各取60只[禽体由内向外预冲洗前（预IOBW）、预冷却和后冷却]。SPCM用于估计预IOBW冲洗液中的沙门氏菌载量，而HGMF用于估计预冷却和后冷却冲洗液中的沙门氏菌水平。还通过富集方法评估了胴体冲洗液中沙门氏菌的流行情况。预IOBW、预冷却和后冷却冲洗液中沙门氏菌的平均流行率分别为95%、100%和41.7%，几何平均载量分别为3.7×10¹、5.6×10⁰和5.0×10⁻²CFU ml⁻¹。
所描述的方法有助于估计家禽胴体冲洗液中活的典型沙门氏菌的浓度。
直接平板计数枚举法有助于在整个生产过程中监测家禽胴体上的沙门氏菌载量，并评估新的加工干预策略。
