Izat A L, Yamaguchi W, Kaniawati S, McGinnis J P, Raymond S G, Hierholzer R E, Kopek J M, Mauromoustakos A
Department of Animal Science, University of Arkansas, Fayetteville 72701.
Poult Sci. 1991 Jun;70(6):1448-51. doi: 10.3382/ps.0701448.
Two similar trials were conducted to evaluate the accuracy of the whole carcass rinse technique in combination with a most probable number (MPN) procedure for estimating the number of salmonellae on postchill broilers. Birds were reared in litter-floored pens and inoculated with Salmonella typhimurium (10(8) cfu/mL) on Days 2, 7, and 14. In each of the two trials six carcasses were consecutively rinsed four times. Each carcass was rinsed with 100 mL of sterile water in sterile plastic bags using an automated shaking device. Salmonellae were enumerated using a three-tube MPN procedure in selenite cystine broth. There were no statistical differences in log10 MPN salmonellae per milliliter of recovered rinse fluid due to trial or consecutive rinse. In several cases salmonellae were not recovered in the initial rinse but were recovered from consecutive rinses of the same carcass. A large amount of variation in MPN levels of salmonellae among individual carcasses occurred within each consecutive rinse. The data suggested that only a percentage of the total salmonellae present on a postchill carcass were recovered with each consecutive rinse, and the organisms were firmly attached prior to processing.
进行了两项类似试验，以评估全胴体冲洗技术结合最大可能数（MPN）程序来估计冷却后肉鸡沙门氏菌数量的准确性。鸡饲养在有垫料的鸡舍中，并在第2、7和14天接种鼠伤寒沙门氏菌（10⁸ cfu/mL）。在两项试验中，每项试验均连续冲洗6只胴体4次。使用自动振荡装置，在无菌塑料袋中用100 mL无菌水冲洗每只胴体。在亚硒酸盐胱氨酸肉汤中使用三管MPN程序对沙门氏菌进行计数。由于试验或连续冲洗，每毫升回收冲洗液中沙门氏菌的log₁₀ MPN没有统计学差异。在一些情况下，初始冲洗未检出沙门氏菌，但同一胴体的连续冲洗中检出了沙门氏菌。在每次连续冲洗中，各个胴体的沙门氏菌MPN水平存在大量差异。数据表明，每次连续冲洗只能回收冷却后胴体上存在的沙门氏菌总数的一定百分比，并且这些微生物在加工前附着牢固。
