Wang Lang, Jiang Hong, Zhu Li-hua, Wen Hua-zhi
Department of Cardiology, Renmin Hospital of Wuhan University, Wuhan 430060, China.
Zhonghua Xin Xue Guan Bing Za Zhi. 2007 Sep;35(9):844-7.
To investigate the effects of endothelial progenitor cells (EPCs) transfusion on reendothelialization and neointima proliferation in balloon-denuded rabbit carotid arteries.
Bone marrow-derived rabbit mononuclear cells (MNCs) were cultured in endothelial basal medium to form EPCs. The cell makers were assayed by immunocytochemistry. The rabbit right carotid artery was injured with 2.5 FPTCA balloon catheter and the EPCs (5 ml) were transfused into the injured carotid artery at a rate of 15 ml/h. The rabbits were killed at 7 days or 14 days post operation. Reendothelialization area and ratio of intima/media (I/M) in injured artery were measured.
EPCs transfusion significantly increased the percentage of endothelialization at 7 days (50.923% +/- 2.476% vs. 28.647% +/- 2.241%) and at 14 days (82.609% +/- 2.611% vs. 49.800% +/- 2.660%) compared to control group (all P < 0.05). I/M ratio was significantly lower in EPCs transfusion group than that in control group (0.378 +/- 0.029 vs. 0.898 +/- 0.038, P < 0.05) 14 days after operation. The labeled EPCs could be detected by immunohistochemistry in the injured vessel wall.
Intra-arterial transfusion of EPCs can effectively accelerate reendothelialization and reduce neointima formation in balloon-denuded rabbit carotid arteries.
探讨输注内皮祖细胞（EPCs）对兔颈动脉球囊损伤后再内皮化及新生内膜增殖的影响。
将兔骨髓来源的单个核细胞（MNCs）在内皮细胞基础培养基中培养以形成EPCs。通过免疫细胞化学检测细胞标志物。用2.5F PTCA球囊导管损伤兔右侧颈动脉，并以15ml/h的速度将EPCs（5ml）输注至损伤的颈动脉。术后7天或14天处死兔子。测量损伤动脉的再内皮化面积及内膜/中膜（I/M）比值。
与对照组相比，输注EPCs后7天（50.923%±2.476% 对 28.647%±2.241%）和14天（82.609%±2.611% 对 49.800%±2.660%）时内皮化百分比显著增加（均P<0.05）。术后14天，EPCs输注组的I/M比值显著低于对照组（0.378±0.029对0.898±0.038，P<0.05）。通过免疫组织化学可在损伤血管壁中检测到标记的EPCs。
动脉内输注EPCs可有效加速兔颈动脉球囊损伤后的再内皮化并减少新生内膜形成。
