Yun Jun-Won, Kim Young-Kyung, Lee Byoung-Seok, Kim Chae-Wook, Hyun Jin-Sook, Baik Joo-Hyun, Kim Jung-Ju, Kim Bae-Hwan
Amorepacific Corporation R&D Center, Yongin-si, Gyeonggi-do, Republic of Korea.
Biosci Biotechnol Biochem. 2007 Dec;71(12):2999-3006. doi: 10.1271/bbb.70403. Epub 2007 Dec 7.
This study was designed to determine whether dietary epigallocatechin-3-gallate (EGCG), the most abundant catechin polyphenol in green tea, can protect the liver from cytochrome P450 2E1 (CYP2E1)-dependent alcoholic liver damage. Compared with an ethanol group, when EGCG was present in the ethanol diet, the formation of a fatty liver was significantly reduced and the serum aspartate transaminase (AST) and alanine transaminase (ALT) levels were much lower. Ethanol treatment significantly elevated hepatic CYP2E1 expression while simultaneously reducing hepatic phospho-acetyl CoA carboxylase (p-ACC) and carnitine palmitoyl-transferase 1 (CPT-1) levels. While EGCG markedly reversed the effect of ethanol on hepatic p-ACC and CPT-1 levels, it had no effect on the ethanol-induced elevation in CYP2E1 expression. EGCG prevents ethanol-induced hepatotoxicity and inhibits the development of a fatty liver. These effects were associated with improvements in p-ACC and CPT-1 levels. The use of EGCG might be useful in treating patients with an alcoholic fatty liver.
本研究旨在确定膳食表没食子儿茶素-3-没食子酸酯（EGCG）——绿茶中含量最丰富的儿茶素多酚——是否能够保护肝脏免受细胞色素P450 2E1（CYP2E1）依赖性酒精性肝损伤。与乙醇组相比，当乙醇饮食中存在EGCG时，脂肪肝的形成显著减少，血清天冬氨酸转氨酶（AST）和丙氨酸转氨酶（ALT）水平也低得多。乙醇处理显著提高肝脏CYP2E1表达，同时降低肝脏磷酸化乙酰辅酶A羧化酶（p-ACC）和肉碱棕榈酰转移酶1（CPT-1）水平。虽然EGCG显著逆转了乙醇对肝脏p-ACC和CPT-1水平的影响，但对乙醇诱导的CYP2E1表达升高没有影响。EGCG可预防乙醇诱导的肝毒性并抑制脂肪肝的发展。这些作用与p-ACC和CPT-1水平的改善有关。使用EGCG可能对治疗酒精性脂肪肝患者有用。
