聚乙二醇诱导浊点系统中微生物转化生产L-苯乙酰基甲醇

Production of L-phenylacetylcarbinol by microbial transformation in polyethylene glycol-induced cloud point system.

作者信息

Zhang Wenzhi, Wang Zhilong, Li Wei, Zhuang Baohua, Qi Hanshi

机构信息

School of Pharmacy, Shanghai Jiao Tong University, Shanghai, 200240, People's Republic of China.

出版信息

Appl Microbiol Biotechnol. 2008 Feb;78(2):233-9. doi: 10.1007/s00253-007-1304-2. Epub 2007 Dec 11.

DOI:10.1007/s00253-007-1304-2

PMID:18071640

Abstract

Microbial transformation of benzaldehyde into L: -phenylacetylcarbinol by whole cell Saccharomyces cerevisiae has been carried out in a novel polyethylene glycol (PEG)-induced cloud point system. The system is composed of 80 g PEG 20,000, 75 ml Triton X-100, 20 g peptone, 10 g yeast extract, 25 g glucose, 1 g MgSO(4).7H(2)O, 0.05 g CaCl(2).2H(2)O, 35 g Na(2)HPO(4).12H(2)O, and 10.7 g citric acid per liter of tap water. The microbial transformation is conducted at 0.6 ml of acetaldehyde (35% volume content), 0.9 ml of benzaldehyde, and 7 g of wet cell per 100 ml of the PEG-induced cloud point system. Under the conditions, a relatively longer-term bioactivity of whole cell microorganism in the PEG-induced cloud point system has been achieved. A fed-batch microbial transformation process with a discrete addition of glucose and substrate gets a high final product concentration of about 8 g/l.

摘要

通过全细胞酿酒酵母将苯甲醛微生物转化为L-苯基乙酰甲醇是在一种新型聚乙二醇（PEG）诱导的浊点系统中进行的。该系统每升自来水中含有80克PEG 20,000、75毫升吐温X-100、20克蛋白胨、10克酵母提取物、25克葡萄糖、1克MgSO₄·7H₂O、0.05克CaCl₂·2H₂O、35克Na₂HPO₄·12H₂O和10.7克柠檬酸。微生物转化在每100毫升PEG诱导的浊点系统中加入0.6毫升乙醛（体积含量35%）、0.9毫升苯甲醛和7克湿细胞的条件下进行。在此条件下，实现了全细胞微生物在PEG诱导的浊点系统中相对较长时间的生物活性。通过分批补料的微生物转化过程，离散添加葡萄糖和底物，最终产物浓度高达约8克/升。