School of Pharmacy, Shanghai Jiao Tong University, Shanghai, People's Republic of China.
Appl Microbiol Biotechnol. 2010 Jan;85(3):517-24. doi: 10.1007/s00253-009-2139-9. Epub 2009 Jul 24.
Extractive microbial transformation of L-phenylacetylcarbinol (L-PAC) in nonionic surfactant Triton X-100 micelle aqueous solution was investigated by response surface methodology. Based on the Box-Behnken design, a mathematical model was developed for the predication of mutual interactions between benzaldehyde, Triton X-100, and glucose on L-PAC production. It indicated that the negative or positive effect of nonionic surfactant strongly depended on the substrate concentration. The model predicted that the optimal concentration of benzaldehyde, Triton X-100, and glucose was 1.2 ml, 15 g, and 2.76 g per 100 ml, respectively. Under the optimal condition, the maximum L-PAC production was 27.6 mM, which was verified by a time course of extractive microbial transformation. A discrete fed-batch process for verification of cell activity was also presented.
采用响应面法研究了非离子表面活性剂 Triton X-100 胶束水溶液中 L-苯乙酰甲醇（L-PAC）的提取微生物转化。基于 Box-Behnken 设计，建立了用于预测苯甲醛、Triton X-100 和葡萄糖对 L-PAC 生产相互作用的数学模型。结果表明，非离子表面活性剂的负或正效应强烈依赖于底物浓度。该模型预测，苯甲醛、Triton X-100 和葡萄糖的最佳浓度分别为 1.2 ml、15 g 和 2.76 g/100 ml。在最佳条件下，L-PAC 的最大产量为 27.6 mM，通过提取微生物转化的时程实验得到验证。还提出了离散进料分批过程以验证细胞活性。
