Ganas Petra, Sachelaru Paula, Mihasan Marius, Igloi Gabor L, Brandsch Roderich
Institute of Biochemistry and Molecular Biology, Centre for Biochemistry and Molecular Cell Biology, Albert-Ludwigs-University Freiburg, Hermann Herder Strasse 7, Freiburg, Germany.
Arch Microbiol. 2008 May;189(5):511-7. doi: 10.1007/s00203-007-0340-8. Epub 2007 Dec 11.
A virtually identical nicotine catabolic pathway including the heterotrimeric molybdenum enzyme nicotine and 6-hydroxy-pseudo-oxynicotine dehydrogenase, 6-hydroxy-L: -nicotine oxidase, 2,6-dihydroxy-pseudo-oxynicotine hydrolase, and 2,6-dihydroxypyridine hydroxylase have been identified in A. nicotinovorans and Nocardioides sp. JS614. Enzymes catalyzing the same reactions and similar protein antigens were detected in the extracts of the two microorganisms. Nicotine blue and methylamine, two end products of nicotine catabolism were detected in the growth medium of both bacterial species. Nicotine catabolic genes are clustered on pAO1 in A. nicotinovorans, but located chromosomally in Nocardioides sp. JS614.
在嗜烟产碱菌和诺卡氏菌属JS614中已鉴定出一条几乎相同的尼古丁分解代谢途径，该途径包括异源三聚体钼酶尼古丁和6-羟基-假氧化尼古丁脱氢酶、6-羟基-L-尼古丁氧化酶、2,6-二羟基-假氧化尼古丁水解酶以及2,6-二羟基吡啶羟化酶。在这两种微生物的提取物中检测到了催化相同反应的酶和相似的蛋白质抗原。在这两种细菌的生长培养基中均检测到了尼古丁分解代谢的两种终产物尼古丁蓝和甲胺。尼古丁分解代谢基因在嗜烟产碱菌中聚集在pAO1质粒上，但在诺卡氏菌属JS614中位于染色体上。
