Hirata Isao, Nomura Yuji, Ito Manabu, Shimazu Atsushi, Okazaki Masayuki
Department of Biomaterials Science, Graduate School of Biomedical Sciences, Hiroshima University, 1-2-3 Kasumi, Minami-ku, Hiroshima 734-8553, Japan.
J Artif Organs. 2007;10(4):212-7. doi: 10.1007/s10047-007-0391-2. Epub 2007 Dec 20.
The development is expected of scaffold biomaterials that feature a shape-maintaining property in addition to high porosity and large pores that cells can easily invade. To develop a new biodegradable scaffold biomaterial reinforced with a frame, synthesized carbonate apatite (CO3Ap) was mixed with neutralized collagen gel, and the CO3Ap-collagen mixtures were lyophilized into sponges in a porous hydroxyapatite (HAp) frame ring. X-ray diffraction and Fourier transform infrared spectroscopy (FT-IR) analyses together with chemical analysis indicated that the synthesized CO3Ap had a crystalline nature and a chemical composition similar to that of bone. Scanning electron microscope (SEM) observation showed that the CO3Ap-collagen sponge had a sui pore size for cell invasion. In proliferation and differentiation experiments with osteoblasts, alkaline phosphatase and osteopontin activity were clearly detected. When these sponge-frame complexes with bone morphogenic protein (rh-BMP2) were implanted beneath the periosteum cranii of rats, significant new bone was created at the surface of the periosteum cranii after 4 weeks of implantation. These reinforced CO3Ap-collagen sponges with rh-BMP2 are expected to be used as hard tissue scaffold biomaterials for the therapeutic purpose of the rapid cure of bone defects.
人们期望开发出一种支架生物材料，它除了具有高孔隙率和大孔隙以便细胞能够轻松侵入外，还具有形状保持特性。为了开发一种用框架增强的新型可生物降解支架生物材料，将合成的碳酸磷灰石（CO3Ap）与中和的胶原蛋白凝胶混合，然后将CO3Ap - 胶原蛋白混合物冻干成海绵状，并置于多孔羟基磷灰石（HAp）框架环中。X射线衍射和傅里叶变换红外光谱（FT - IR）分析以及化学分析表明，合成的CO3Ap具有晶体性质且化学成分与骨相似。扫描电子显微镜（SEM）观察显示，CO3Ap - 胶原蛋白海绵具有适合细胞侵入的孔径。在成骨细胞的增殖和分化实验中，清晰检测到了碱性磷酸酶和骨桥蛋白活性。当将这些与骨形态发生蛋白（rh - BMP2）结合的海绵框架复合物植入大鼠颅骨骨膜下时，植入4周后在颅骨骨膜表面产生了大量新骨。这些含有rh - BMP2的增强型CO3Ap - 胶原蛋白海绵有望用作硬组织支架生物材料，用于快速治愈骨缺损的治疗目的。
