Benimeli C S, González A J, Chaile A P, Amoroso M J
PROIMI-CONICET, Av. Belgrano y Caseros, Tucumán, Argentina.
J Basic Microbiol. 2007 Dec;47(6):468-73. doi: 10.1002/jobm.200700242.
This work was conducted to study the removal of gamma-hexachlorocyclohexane (lindane) in a soil extract liquid medium (SE) by Streptomyces sp. M7 and to determine the influence of pH and temperature on bacterial growth and pesticide removal in this medium. When Streptomyces sp. M7 was cultured in SE supplemented with lindane 100 microg l(-1 )at different initial pH, the maximum growth was observed at pH 7 and the microorganism was not able to grow at pH 5 and 9; the highest pesticide removal (70.4%) by Streptomyces sp. M7 was noted at an initial pH of 7 at 4 weeks of incubation. The maximum removal (70% approximately) was observed when the microorganism was incubated in SE at 30 degrees C; although the optimal temperature for Streptomyces sp. M7 growth, with and without lindane, was 25 degrees C, and for the pesticide removal was 30 degrees C. The results of this study suggest that this actinomycete strain appears as an effective alternative in the remediation of lindane polluted sites.
开展这项工作是为了研究链霉菌属M7在土壤提取液培养基（SE）中对γ-六氯环己烷（林丹）的去除情况，并确定pH值和温度对该培养基中细菌生长及农药去除的影响。当链霉菌属M7在添加了100微克/升林丹的不同初始pH值的SE培养基中培养时，在pH值为7时观察到最大生长量，而该微生物在pH值为5和9时无法生长；在初始pH值为7、培养4周时，链霉菌属M7对农药的去除率最高（70.4%）。当微生物在30℃的SE培养基中培养时，观察到最大去除率（约70%）；尽管链霉菌属M7生长的最佳温度，无论有无林丹存在，均为25℃，但农药去除的最佳温度为30℃。本研究结果表明，这种放线菌菌株似乎是修复林丹污染场地的一种有效替代方法。
