Mondoux Michelle A, Zakian Virginia A
Department of Molecular Biology, Princeton University, Princeton, New Jersey 08544, USA.
Genetics. 2007 Dec;177(4):2541-6. doi: 10.1534/genetics.107.079806.
In Saccharomyces cerevisiae, genes placed near telomeres are transcriptionally repressed (telomere position effect, TPE). Although telomeric DNA sequence is the same at all chromosome ends, the subtelomeric elements (STEs) and level of TPE vary from telomere to telomere. We tested whether STEs determine TPE levels. STEs contributed to TPE, as deleting the X element from the VI-R telomere modestly decreased silencing at this telomere. However, STEs were not the major determinant of TPE levels, as inserting the VI-R X element at the truncated VII-L telomere did not increase TPE. These data suggest that the TPE levels of individual telomeres are dependent on some aspect of chromosome context.
在酿酒酵母中，位于端粒附近的基因会受到转录抑制（端粒位置效应，TPE）。尽管所有染色体末端的端粒DNA序列相同，但亚端粒元件（STE）和TPE水平在不同端粒之间存在差异。我们测试了STE是否决定TPE水平。STE对TPE有贡献，因为从VI-R端粒删除X元件会适度降低该端粒处的沉默。然而，STE不是TPE水平的主要决定因素，因为在截短的VII-L端粒插入VI-R X元件并没有增加TPE。这些数据表明，单个端粒的TPE水平取决于染色体背景的某些方面。
