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[Cross-linking of crystalline sperm whale myoglobin using glutaraldehyde].

作者信息

Artiukh R I, Kachalova G S, Lanina N F, Miachin E T

出版信息

Biokhimiia. 1991 Dec;56(12):2140-7.

PMID:1807403
Abstract

The salt composition of the solution has been found, which does not interact with glutaraldehyde and myoglobin at an ionic strength corresponding to that of the mother liquid, i.e., 4.5 M (NH4)2SO4. Cross-linking conditions have been elaborated when crystalline myoglobin retains its native structure: myoglobin in 3 M Cs2SO4 pH 5.4 is cross-linked by diffusion in 5% glutaraldehyde vapours in the same salt solution (5 days, room temperature).

摘要

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