Gao Shumin, Wen Xiaohui, Yang Rongrong, Di Keqian, Tong Jun, Li Xiangyun
College of Animal Science and Technology, Agricultural University of Hebei, Baoding, Hebei, China.
In Vitro Cell Dev Biol Anim. 2008 Jan-Feb;44(1-2):10-6. doi: 10.1007/s11626-007-9070-0. Epub 2007 Dec 11.
ES mice that are derived completely from embryonic stem (ES) cells can be obtained by tetraploid embryo complementation. Many neonate ES mice die because of respiratory distress, but it is not clear what contributes to the phenomenon. Using five microsatellite DNA markers, we confirmed that our ES mice were completely derived from ES cells and contained no tetraploid component. The neonatal ES mice that exhibited respiratory distress were tested for surfactant protein B (SP-B) expression by Western blotting. These mice had no SP-B expression, and even apparently healthy adult ES mice had decreased SP-B levels and aberrant SP-B phenotypes. These data suggest that the expression of SP-B protein is an important factor in the survival of ES mice to term and adulthood.
完全由胚胎干细胞（ES细胞）发育而来的ES小鼠可通过四倍体胚胎互补技术获得。许多新生ES小鼠因呼吸窘迫而死亡，但不清楚导致这一现象的原因。我们使用五个微卫星DNA标记，证实我们的ES小鼠完全源自ES细胞，不含四倍体成分。通过蛋白质免疫印迹法检测出现呼吸窘迫的新生ES小鼠的表面活性蛋白B（SP-B）表达情况。这些小鼠没有SP-B表达，甚至明显健康的成年ES小鼠也有SP-B水平降低和异常的SP-B表型。这些数据表明，SP-B蛋白的表达是ES小鼠存活至足月和成年的一个重要因素。
