酪氨酸激酶抑制剂染料木黄酮和赫比霉素对牛肾上腺嗜铬细胞儿茶酚胺释放的影响。
Effects of genistein and herbimycin, tyrosine kinase inhibitors, on catecholamine release in bovine adrenal chromaffin cells.
作者信息
Matsumura C, Kuwashima H, Soma S, Kimura T
机构信息
Department of Pharmacology, The Nippon Dental University School of Life Dentistry at Niigata, Hamaura-cho, Chuou-ku, Niigata 951-8580, Japan.
出版信息
Auton Autacoid Pharmacol. 2007 Oct;27(4):181-7. doi: 10.1111/j.1474-8673.2007.00411.x.
1 The effects of genistein and herbimycin, tyrosine kinase inhibitors, on catecholamine (CA) release were examined in bovine adrenal chromaffin cells. 2 In intact cells, genistein (10-100 microm) and herbimycin (3-30 microm) inhibited CA release induced by acetylcholine (ACh; 100 microm) or the nicotinic receptor stimulant 1,1-dimethyl-4-phenyl-piperazinium (DMPP; 10 microm), but did not affect CA release induced by high K+ (40 mm). 3 Genistein and herbimycin inhibited (45)Ca2+ uptake induced by ACh (100 microm). 4 Neither genistein nor herbimycin affected [(3)H]nicotine binding with nicotinic receptors. 5 In beta-escin-permeabilized cells, neither genistein nor herbimycin affected CA release induced by Ca2+ (1 microm). 6 These results suggest that protein tyrosine kinase plays the facilitatory role in the regulation of CA release induced by nicotinic receptor stimulation in stimulus-secretion coupling of bovine adrenal chromaffin cells.
- 研究了酪氨酸激酶抑制剂染料木黄酮和赫曲霉素对牛肾上腺嗜铬细胞儿茶酚胺（CA）释放的影响。2. 在完整细胞中，染料木黄酮（10 - 100微摩尔）和赫曲霉素（3 - 30微摩尔）抑制由乙酰胆碱（ACh；100微摩尔）或烟碱受体激动剂1,1 - 二甲基 - 4 - 苯基哌嗪鎓（DMPP；10微摩尔）诱导的CA释放，但不影响由高钾（40毫摩尔）诱导的CA释放。3. 染料木黄酮和赫曲霉素抑制由ACh（100微摩尔）诱导的（45）Ca2 +摄取。4. 染料木黄酮和赫曲霉素均不影响[（3）H]尼古丁与烟碱受体的结合。5. 在β - 七叶皂苷通透的细胞中，染料木黄酮和赫曲霉素均不影响由Ca2 +（1微摩尔）诱导的CA释放。6. 这些结果表明，在牛肾上腺嗜铬细胞的刺激 - 分泌偶联中，蛋白酪氨酸激酶在烟碱受体刺激诱导的CA释放调节中起促进作用。
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