Wang C M, Lo L C, Feng F, Zhu Z Y, Yue G H
Molecular Population Genetics Group, Temasek Life Sciences Laboratory, 1 Research Link, National University of Singapore, 117604 Singapore.
Anim Genet. 2008 Feb;39(1):34-9. doi: 10.1111/j.1365-2052.2007.01672.x. Epub 2007 Dec 12.
Quantitative trait loci (QTL) affecting growth traits have previously been mapped in linkage groups (LG) 2, 3 and 23 of Barramundi (Lates carcalifer), but these QTL have not been verified in different genetic backgrounds and environments. Here, we report the identification and verification of QTL for growth traits on LG2, 3, 10 and 23 in F(1) families constructed using brooders from the Singapore Marine Aquaculture Center (MAC) and from wild stocks collected in Thailand (THAI). The previously detected QTL for body weight and length linked to marker Lca371 on LG2 were confirmed in both the MAC and THAI families, whereas other QTL previously mapped to LG3 and 23 were only detected in one of the two families. QTL for body weight and length were identified in the MAC family, but not in the THAI family, in a region where the insulin-like growth factor 2 (IGF2) and tyrosine hydroxylase 1 (TH1) genes are located on LG10. Significant epistatic interactions were identified between markers Lca287 on LG2 and IGF2 on LG10 for growth trait QTL in the MAC family, but not in the THAI family. Effects of the IGF2, TH1 and parvalbumin 1 candidate genes were family-specific. Our results indicate that some but not all QTL are family-specific in Barramundi.
影响生长性状的数量性状位点（QTL）先前已在尖吻鲈（Lates calcarifer）的连锁群（LG）2、3和23中定位，但这些QTL尚未在不同的遗传背景和环境中得到验证。在此，我们报告了在使用来自新加坡海洋水产养殖中心（MAC）的亲鱼和从泰国收集的野生种群（THAI）构建的F(1)家系中，对LG2、3、10和23上生长性状QTL的鉴定和验证。先前检测到的与LG2上标记Lca371连锁的体重和体长QTL在MAC和THAI家系中均得到证实，而先前定位到LG3和23的其他QTL仅在两个家系中的一个中检测到。在MAC家系中鉴定出了体重和体长的QTL，但在THAI家系中未鉴定出，该QTL位于LG10上胰岛素样生长因子2（IGF2）和酪氨酸羟化酶1（TH1）基因所在的区域。在MAC家系中，LG2上的标记Lca287与LG10上的IGF2之间存在显著的生长性状QTL上位性相互作用，但在THAI家系中未发现。IGF2、TH1和小白蛋白1候选基因的效应具有家系特异性。我们的结果表明，尖吻鲈中的一些但并非所有QTL都具有家系特异性。
