Haidar Ziyad S, Hamdy Reggie C, Tabrizian Maryam
Faculty of Dentistry, McGill University, Montréal, Québec, Canada.
Biomaterials. 2008 Mar;29(9):1207-15. doi: 10.1016/j.biomaterials.2007.11.012.
The present work is focused on the formulation of core-shell nanoparticles via the layer-by-layer (L-b-L) self-assembly technique for delivery of biomacromolecules such as bone growth factors. The drug encapsulation efficiency of liposomes is enhanced with the increased stability of polyelectrolyte systems achieved through the alternate adsorption of several layers of natural polymers: anionic alginate and cationic chitosan on cationic nanosized phospholipid vesicles. The resulting particles were characterized for their size, surface charge, morphology, encapsulation efficiency, loading capacity and release kinetics over an extended period of 30 days. The L-b-L deposition technique succeeded in building a spherical, monodisperse and stable hybrid nanoparticulate protein delivery system with a cumulative size of 383+/-11.5 nm and zeta potential surface charge of 44.61+/-3.31 mV for five bilayered liposomes. The system offers numerous compartments for encapsulation including the aqueous core and within the polyelectrolyte shell demonstrating good entrapment and sustained linear release of a model protein, bovine serum albumin, in vitro. Our results demonstrate that this delivery system features an extended shelf life and can be loaded immediately prior to administration, thus preventing any loss of the protein.
本研究致力于通过层层（L-b-L）自组装技术制备核壳纳米颗粒，用于递送诸如骨生长因子等生物大分子。通过在阳离子纳米磷脂囊泡上交替吸附几层天然聚合物（阴离子藻酸盐和阳离子壳聚糖），提高了聚电解质系统的稳定性，从而增强了脂质体的药物包封效率。对所得颗粒的大小、表面电荷、形态、包封效率、载药量和长达30天的释放动力学进行了表征。层层沉积技术成功构建了一种球形、单分散且稳定的混合纳米颗粒蛋白递送系统，对于五层双层脂质体，其累积大小为383±11.5 nm，ζ电位表面电荷为44.61±3.31 mV。该系统提供了多个包封隔室，包括水相核心和聚电解质壳层内，在体外对模型蛋白牛血清白蛋白表现出良好的截留和持续线性释放。我们的结果表明，这种递送系统具有较长的保质期，并且可以在给药前立即装载，从而防止蛋白质的任何损失。
