小鼠和鸡胚胎发育过程中Lin-28的动态基因表达。
Dynamic gene expression of Lin-28 during embryonic development in mouse and chicken.
作者信息
Yokoyama Shigetoshi, Hashimoto Megumi, Shimizu Hirohito, Ueno-Kudoh Hiroe, Uchibe Kenta, Kimura Ichiro, Asahara Hiroshi
机构信息
Department of Systems Biomedicine, National Research Institute for Child Health and Development, 2-10-1 Okura, Setagaya, Tokyo, Japan.
出版信息
Gene Expr Patterns. 2008 Feb;8(3):155-60. doi: 10.1016/j.gep.2007.11.001. Epub 2007 Nov 17.
The Caenorhabditis elegans heterochronic gene lin-28 regulates developmental timing in the nematode trunk. We report the dynamic expression patterns of Lin-28 homologues in mouse and chick embryos. Whole mount in situ hybridization revealed specific and intriguing expression patterns of Lin-28 in the developing mouse and chick limb bud. Mouse Lin-28 expression was detected in both the forelimb and hindlimb at E9.5, but disappeared from the forelimb at E10.5, and finally from the forelimb and hindlimb at E11.5. Chicken Lin-28, which was first detected in the limb primordium at stage 15/16, was also downregulated as the stage proceeded. The amino acid sequences of mouse and chicken Lin-28 genes are highly conserved and the similar expression patterns of Lin-28 during limb development in mouse and chicken suggest that this heterochronic gene is also conserved during vertebrate limb development.
秀丽隐杆线虫异时性基因lin - 28调控线虫躯干的发育时间。我们报道了Lin - 28同源物在小鼠和鸡胚胎中的动态表达模式。全胚胎原位杂交揭示了Lin - 28在发育中的小鼠和鸡肢体芽中的特异性和有趣的表达模式。在E9.5时，小鼠Lin - 28在前肢和后肢均有表达，但在E10.5时在前肢消失，最终在E11.5时在前肢和后肢均消失。鸡Lin - 28在第15/16阶段首次在肢体原基中被检测到，随着发育阶段的推进也被下调。小鼠和鸡Lin - 28基因的氨基酸序列高度保守，并且Lin - 28在小鼠和鸡肢体发育过程中的相似表达模式表明，这种异时性基因在脊椎动物肢体发育过程中也是保守的。
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