通过单分子光谱对沼泽红假单胞菌RC LH1核心复合物X射线结构的优化。
Refinement of the x-ray structure of the RC LH1 core complex from Rhodopseudomonas palustris by single-molecule spectroscopy.
作者信息
Richter Martin F, Baier Jürgen, Southall June, Cogdell Richard J, Oellerich Silke, Köhler Jörgen
机构信息
Experimental Physics IV and Bayreuth Institute for Macromolecular Research, Universität Bayreuth, Universtitätsstrasse 30, D-95440 Bayreuth, Germany.
出版信息
Proc Natl Acad Sci U S A. 2007 Dec 18;104(51):20280-4. doi: 10.1073/pnas.0704599105. Epub 2007 Dec 12.
Abstract
A unique combination of single-molecule spectroscopy with numerical simulations has allowed us to achieve a refined structural model for the bacteriochlorophyll a (BChl a) pigment arrangement in reaction center-light-harvesting 1 core complexes of Rhodopseudomonas palustris. Details in the optical spectra, such as spectral separation and mutual polarizations of spectral bands, are compared with results from numerical simulations for various models of the BChl a arrangement that were all well within the 4.8-A limit of the accuracy of the available x-ray structure. The experimental data are consistent with a geometry where 15 BChl a dimers, each taken homologous to those from light-harvesting 2 complex from Rhodopseudomonas acidophila, are arranged in an overall elliptical structure featuring a gap on the long side of the ellipse.
摘要
单分子光谱学与数值模拟的独特结合，使我们能够为沼泽红假单胞菌反应中心-光捕获1核心复合物中的细菌叶绿素a（BChl a）色素排列建立一个精细的结构模型。将光谱中的细节，如光谱带的光谱分离和相互偏振，与各种BChl a排列模型的数值模拟结果进行比较，这些模型都在可用X射线结构精度的4.8埃限制范围内。实验数据与这样一种几何结构一致，即15个BChl a二聚体（每个二聚体与嗜酸红假单胞菌光捕获2复合物中的二聚体同源）排列成一个整体椭圆形结构，在椭圆长边上有一个间隙。
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