缺氧诱导因子-1α过表达增加LNCaP细胞的血管生成
[Over-expression of hypoxia-inducible factor-1 alpha increases angiogenesis of LNCaP cells].
作者信息
Han Yi-li, He Da-lin, Luo Yong, Cheng He-peng, Zhu Guang-feng
机构信息
Department of Urology, the First Hospital of Xi'an Jiaotong University, Xi'an, Shaanxi 710061, China.
出版信息
Zhonghua Nan Ke Xue. 2007 Nov;13(11):988-91.
OBJECTIVE
To evaluate the effect of hypoxia-inducible factor-1 alpha (HIF-la) on angiogenesis in human prostate cancer cells.
METHODS
Human prostate cancer cells of the line LNCaP were cultured and transfected by the recombinant plasmid pcDNA3. 1(-)/HIF-1alpha containing the gene HIF-1alpha with the Lipofectamine 2000 system. The positive clone cells were selected by G418 and confirmed by Western blotting and immunofluorescence (LNCaP/HIF-1alpha cells). The expressions of VEGF, iNOS and Ang-2 were detected by Western blotting.
RESULTS
The expression of HIF-1alpha in the LNCaP/HIF-1alpha cells was distinctly higher than that in the LN-CaP cells. Compared with the LNCaP cells, the expressions of VEGF and iNOS were up-regulated, whereas Ang-2 remained unchanged in the LNCaP/HIF-1alpha cells.
CONCLUSION
The over-expression of HIF-1alpha can induce an increase in angiogenesis proteins and improve the angiogenesis potency of prostate cancer.
目的
评估缺氧诱导因子-1α（HIF-1α）对人前列腺癌细胞血管生成的影响。
方法
培养人前列腺癌LNCaP细胞系，并用脂质体2000系统将含HIF-1α基因的重组质粒pcDNA3.1(-)/HIF-1α转染至细胞。通过G418筛选阳性克隆细胞，并经蛋白质免疫印迹法和免疫荧光法确认（LNCaP/HIF-1α细胞）。采用蛋白质免疫印迹法检测VEGF、iNOS和Ang-2的表达。
结果
LNCaP/HIF-1α细胞中HIF-1α的表达明显高于LNCaP细胞。与LNCaP细胞相比，LNCaP/HIF-1α细胞中VEGF和iNOS的表达上调，而Ang-2的表达无变化。
结论
HIF-1α的过表达可诱导血管生成相关蛋白增加，提高前列腺癌的血管生成能力。
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