[前列腺癌抗原-1在前列腺癌中的表达及其临床意义]

[Expression of prostate cancer antigen-1 in prostate cancer and its clinical significance].

作者信息

Liu Bing-qian, Wu Yu-dong, Wei Jin-xing, Zhang Tong, Liu Ran-lu, Ma Teng-xiang

机构信息

Department of Urology, the First Affiliated Hospital of Zhengzhou University, Zhengzhou, Henan 450052, China.

出版信息

Zhonghua Nan Ke Xue. 2007 Nov;13(11):997-1001.

PMID:18077911

Abstract

OBJECTIVE

To investigate the expression of prostate cancer antigen-1 (PCA-1) in different prostate tissues and analyze its correlation with the clinical parameters of prostate cancer (PCa).

METHODS

The expression of PCA-1 mRNA was detected by RT-PCR in the samples from 45 cases of PCa with various clinico-pathologic characteristics, 30 cases of high-grade prostatic intraepithelial neoplasia (HG-PIN), 43 cases of BPH and 39 cases of other carcinoma tissues. The correlation of PCA-1 mRNA expression with the clinical parameters of PCa was statistically analyzed and the PCA-1 expression was examined in different samples by immunohistochemistry.

RESULTS

The positive expression rate of PCA-1 mRNA was 88.9% and 60.0% and that of PCA-1 protein was 84.4% and 50.0% in the patients with PCa and HG-PIN, respectively. PCA-1 mRNA and PCA-1 proteins were not expressed in the BPH and other carcinoma tissues. The expression of PCA-1 mRNA was unrelated with the clinical parameters of PCa (P > 0.05).

CONCLUSION

It is suggested that PCA-1 is a PCa-specific gene and its expression is unrelated to the clinical parameters of PCa. It might serve as a specific biomarker for the early diagnosis of PCa.

摘要

目的

研究前列腺癌抗原-1（PCA-1）在不同前列腺组织中的表达情况，并分析其与前列腺癌（PCa）临床参数的相关性。

方法

采用逆转录聚合酶链反应（RT-PCR）检测45例具有不同临床病理特征的PCa患者、30例高级别前列腺上皮内瘤变（HG-PIN）患者、43例良性前列腺增生（BPH）患者及39例其他癌组织样本中PCA-1 mRNA的表达。对PCA-1 mRNA表达与PCa临床参数的相关性进行统计学分析，并采用免疫组织化学方法检测不同样本中PCA-1的表达。