Neves Adriana F, Araújo Thaise G, Biase Weruska K F S, Meola Juliana, Alcântara Tânia M, Freitas Danielo G, Goulart Luiz R
Laboratory of Nanobiotechnology, Institute of Genetics and Biochemistry, Federal University of Uberlandia, Uberlandia, Minas Gerais, Brazil.
Clin Biochem. 2008 Oct;41(14-15):1191-8. doi: 10.1016/j.clinbiochem.2008.06.013. Epub 2008 Jul 2.
To develop a semi-quantitative method for prostate cancer diagnosis and to validate this technique in clinical protocols with the use of multiplex RT-PCR assays for five different biomarkers associated with carcinogenesis, including the PCA3 gene.
AR, SRD5A2, KLK2, PSMA, and PCA3 transcripts were analyzed by multiplex RT-PCR assay in 73 prostatic tissue samples from patients with prostate cancer (PCa) and benign hyperplasia (BPH).
Significant differences were observed between cancerous and hypertrophic tissues in the relative expression of these genes. AR, KLK2, PSMA, and PCA3 genes displayed increased transcriptional levels in the cancer specimens; on the other hand, SRD5A2 mRNA levels were higher in the BPH samples.
Our results suggest that the most promising marker for PCa diagnosis was positive PCA3 detection associated with serum PSA levels, which showed 28-fold higher chances for cancer occurrence, with 92% specificity and 94% positive predictive value.
开发一种用于前列腺癌诊断的半定量方法，并通过对包括PCA3基因在内的五种与致癌作用相关的不同生物标志物进行多重逆转录聚合酶链反应（RT-PCR）分析，在临床方案中验证该技术。
通过多重RT-PCR分析，对73例前列腺癌（PCa）患者和良性前列腺增生（BPH）患者的前列腺组织样本中的雄激素受体（AR）、5α-还原酶2（SRD5A2）、激肽释放酶2（KLK2）、前列腺特异性膜抗原（PSMA）和PCA3转录本进行分析。
在这些基因的相对表达中，癌组织和增生组织之间观察到显著差异。AR、KLK2、PSMA和PCA3基因在癌组织标本中的转录水平升高；另一方面，SRD5A2 mRNA水平在BPH样本中更高。
我们的结果表明，前列腺癌诊断最有前景的标志物是与血清前列腺特异性抗原（PSA）水平相关的PCA3阳性检测，其显示癌症发生几率高28倍，特异性为92%，阳性预测值为94%。
