再生障碍性贫血患者骨髓微血管密度及血管内皮生长因子表达

[Bone marrow microvessel density and vascular endothelial growth factor expression in patients with aplastic anemia].

作者信息

Zhang Li, Wang Hui-jun, Li Hong-qiang, Yang Dong-lin, Yan Zhang-song, Wu Yu-hong, Zhou Kang, Chu Yu-lin, Chen Hui-shu, Zhang Feng-kui

机构信息

Institute of Hematology and Blood Diseases Hospital, CAMS & PUMC, Tianjin, China.

出版信息

Zhonghua Xue Ye Xue Za Zhi. 2007 Aug;28(8):528-31.

PMID:18078128

Abstract

OBJECTIVE

To study the bone marrow microvessel density (MVD) and vascular endothelial growth factor (VEGF) expression and their clinical significance in patients with aplastic anemia (AA).

METHODS

Bone marrow biopsies in 51 newly diagnosed patients with AA were evaluated the MVD and VEGF expression by immunostaining with anti-factor VIII related antigen and VEGF monoclonal antibodies at regular time points after immunosuppressive therapy (IT).

RESULTS

The mean bone marrow MVD in AA group was 5.5 +/- 3.5, being significantly lower than that in normal control group (8.7 +/- 3.4, P < 0.05). MVDs of SAA and NSAA patients were 7.4 +/- 2.9 and 4.3 +/- 3.4, respectively, being significantly different (P < 0.01). The VEGF expression in AA group was significantly lower than that in control group [(6.7 +/- 8.4)% vs (14.7 +/- 6.1)%, P < 0.01], but there was no difference between SAA and NSAA. Bone marrow MVD and VEGF were significantly increased after IT in 22 responded AA patients.

CONCLUSION

Bone marrow MVD and VEGF expression are low in AA patients which may be one of pathophysiologic mechanisms of bone marrow failure in AA. Proangiogenic and ameliorating microcirculation agents together with IT might accelerate the recovery of hematopoiesis in AA patients.

摘要

目的

研究再生障碍性贫血（AA）患者的骨髓微血管密度（MVD）和血管内皮生长因子（VEGF）表达及其临床意义。

方法

对51例新诊断的AA患者进行骨髓活检，在免疫抑制治疗（IT）后的定期时间点，用抗VIII因子相关抗原和VEGF单克隆抗体进行免疫染色，评估MVD和VEGF表达。

结果

AA组骨髓平均MVD为5.5±3.5，显著低于正常对照组（8.7±3.4，P<0.05）。重型再生障碍性贫血（SAA）和非重型再生障碍性贫血（NSAA）患者的MVD分别为7.4±2.9和4.3±3.4，差异有统计学意义（P<0.01）。AA组VEGF表达显著低于对照组[（6.7±8.4）%对（14.7±6.1）%，P<0.01]，但SAA和NSAA之间无差异。22例有反应的AA患者IT后骨髓MVD和VEGF显著增加。