多层纳米薄膜中多肽结构变化的可逆性

Reversibility of structural changes of polypeptides in multilayer nanofilms.

作者信息

Zhang Ling, Haynie Donald T

机构信息

Artificial Cell Technologies, Inc., 5 Science Park at Yale, Third Floor, New Haven, Connecticut 06511, USA.

出版信息

Biomacromolecules. 2008 Jan;9(1):185-91. doi: 10.1021/bm700967k. Epub 2007 Dec 14.

DOI:10.1021/bm700967k

PMID:18078320

Abstract

Structural properties of different polypeptide multilayer nanofilms fabricated at neutral pH have been analyzed by UV spectroscopy, circular dichroism spectroscopy (CD), and Fourier-transform infrared spectroscopy (FTIR). The various peptides studied exhibit a strong tendency to adopt a beta sheet conformation in the films. Changes in film structure on dehydration are completely reversed on rewetting. The time scale of reversibility is, however, substantially shorter for the polymer backbone than the side chains, as in protein folding.

摘要

通过紫外光谱、圆二色光谱（CD）和傅里叶变换红外光谱（FTIR）分析了在中性pH条件下制备的不同多肽多层纳米薄膜的结构特性。所研究的各种肽在薄膜中呈现出强烈的形成β折叠构象的趋势。脱水时薄膜结构的变化在重新润湿时完全逆转。然而，与蛋白质折叠情况一样，聚合物主链的可逆时间尺度比侧链短得多。