Zhang Ling, Zhao Wanhua, Rudra Jai S, Haynie Donald T
Artificial Cell Technologies, Inc., 5 Science Park at Yale, Third Floor, New Haven, Connecticut 06511, USA.
ACS Nano. 2007 Dec;1(5):476-86. doi: 10.1021/nn700267g.
Polyelectrolyte multilayer nanofilms and nanocomposites have shown considerable promise for the rational development of multifunctional materials with wide-ranging properties. Polypeptides are a distinctive and largely unexplored class of polyelectrolytes in this context. Methods now exist for the synthesis of peptides with control at the level of the amino acid sequence, and for the preparation of these polymers in massive quantities. Here, we analyze the roles of six designed 32mer peptides in the fabrication, structure, and stability of multilayer nanofilms prepared by layer-by-layer self-assembly. The data show that amino acid sequence and the specific combination of anionic and cationic peptides together have a marked impact on nanofilm growth behavior, secondary structure content, and density in experimental studies. The same factors determine physical properties of the corresponding interpolypeptide complexes in molecular dynamics simulations.
聚电解质多层纳米薄膜和纳米复合材料在合理开发具有广泛性能的多功能材料方面已显示出巨大的潜力。在这种情况下，多肽是一类独特且在很大程度上未被探索的聚电解质。现在有方法可以在氨基酸序列水平上进行控制来合成肽，并且可以大量制备这些聚合物。在这里，我们分析了六种设计的32聚体肽在通过逐层自组装制备的多层纳米薄膜的制造、结构和稳定性中的作用。数据表明，在实验研究中，氨基酸序列以及阴离子和阳离子肽的特定组合对纳米薄膜的生长行为、二级结构含量和密度有显著影响。在分子动力学模拟中，相同的因素决定了相应的多肽间复合物的物理性质。
