Vranesić B, Ljevaković D, Tomasić J, Ladesić B
Department of Radioimmunology, Institute of Immunology, Zagreb, Croatia, Yugoslavia.
Clin Chim Acta. 1991 Oct 14;202(1-2):23-37. doi: 10.1016/0009-8981(91)90252-8.
The preparation and characterization of the essential components to be used in the radioimmunoassay of peptidoglycan monomer (PGM) is described. In order to raise the anti-peptidoglycan monomer antibodies 14C-labelled peptidoglycan monomer-bovine serum albumin conjugate was prepared by the coupling of 14C-peptidoglycan monomer to bovine serum albumin in the presence of glutaraldehyde in 0.1 M NaHCO3 at pH 8.3. The prepared conjugate elicited anti-PGM response in rabbits. A synthetic analog of peptidoglycan monomer, Boc-L-tyrosyl-peptidoglycan monomer was prepared by condensation of unprotected peptidoglycan monomer and N-hydroxysuccinimidester of Boc-L-tyrosine in the presence of triethylamine and the obtained disaccharide-hexapeptide was labelled with Na125I. This compound exhibited the ability of binding to anti-peptidoglycan monomer antibodies. The prepared compounds, namely anti-PGM antibodies and 125I-labelled Boc-L-tyrosyl-peptidoglycan monomer, were used as essential components in competitive radioimmunoassay for peptidoglycan monomer determination in mammalian and human sera and plasma, respectively.
本文描述了用于肽聚糖单体（PGM）放射免疫分析的关键成分的制备与表征。为制备抗肽聚糖单体抗体，在pH 8.3的0.1 M碳酸氢钠中，于戊二醛存在的条件下，将14C标记的肽聚糖单体与牛血清白蛋白偶联，制备了14C标记的肽聚糖单体 - 牛血清白蛋白偶联物。所制备的偶联物在兔体内引发了抗PGM反应。通过在三乙胺存在的条件下，将未保护的肽聚糖单体与Boc-L-酪氨酸的N-羟基琥珀酰亚胺酯缩合，制备了肽聚糖单体的合成类似物Boc-L-酪氨酰 - 肽聚糖单体，并用Na125I对所得的二糖 - 六肽进行标记。该化合物表现出与抗肽聚糖单体抗体结合的能力。所制备的化合物，即抗PGM抗体和125I标记的Boc-L-酪氨酰 - 肽聚糖单体，分别用作放射免疫竞争分析的关键成分，用于测定哺乳动物血清和人血清及血浆中的肽聚糖单体。
