Dong Hua, Cao Xiaodong, Li Chang Ming, Hu Weihua
School of Chemical & Biomedical Engineering, Center for Advanced Bionanosystems, Nanyang Technological University, Singapore, Singapore.
Biosens Bioelectron. 2008 Feb 28;23(7):1055-62. doi: 10.1016/j.bios.2007.10.026. Epub 2007 Nov 6.
A novel in situ electrochemical surface plasmon resonance (EC-SPR) immunosensor is presented in this paper. The EC-SPR measurement can be used to in situ monitor the polymer formation, probe immobilization, antigen-antibody interaction and protein immunosensing process. A sandwich immunosensor based on permeable polypyrrole propylic acid (PPA) film is constructed using mouse IgG as a model analyte. The results show that the introduction of capture antibody conjugated enzyme not only enhances the current responses but also increases the SPR angle shift. The calibration curves of electrochemical (EC) and surface plasmon resonance (SPR) measurement exhibit a similar dependence on the bulk concentration of antigen. An approximate linear relationship can be obtained by plotting the data in semi-logarithmic reference frame. Compared with SPR, EC shows higher sensitivity with prolonged time. The in situ EC-SPR immunosensor described herein could have important potentials for diagnostics and medicine applications.
本文提出了一种新型的原位电化学表面等离子体共振（EC-SPR）免疫传感器。EC-SPR测量可用于原位监测聚合物形成、探针固定、抗原-抗体相互作用及蛋白质免疫传感过程。以小鼠IgG作为模型分析物，构建了基于可渗透聚吡咯丙酸（PPA）膜的夹心免疫传感器。结果表明，引入与捕获抗体偶联的酶不仅增强了电流响应，还增加了SPR角位移。电化学（EC）和表面等离子体共振（SPR）测量的校准曲线对抗原本体浓度呈现相似的依赖性。通过在半对数参考系中绘制数据可得到近似线性关系。与SPR相比，EC在延长时间时显示出更高的灵敏度。本文所述的原位EC-SPR免疫传感器在诊断和医学应用方面可能具有重要潜力。
