Cheng Congsheng, Dong Jie, Yao Lihong, Chen Aijun, Jia Runqing, Huan Lifang, Guo Jianqiang, Shu Yuelong, Zhang Zhiqing
State Key Laboratory for Infectious Disease Prevention and Control, National Institute for Viral Disease Control and Prevention, Chinese Center for Disease Control and Prevention, 100 Yingxin Jie, Xuanwu District, 100052 Beijing, China.
Biochem Biophys Res Commun. 2008 Feb 15;366(3):670-4. doi: 10.1016/j.bbrc.2007.11.183. Epub 2007 Dec 17.
New therapeutics are urgently needed for the treatment of pandemic influenza caused by H5N1 influenza virus mutants. Aptamer was a promising candidate for treatment and prophylaxis of influenza virus infections. In this study, systemic evolution of ligands through exponential enrichment (SELEX) was used to screen DNA aptamers targeted to recombinant HA1 proteins of the H5N1 influenza virus. After 11 rounds of selection, DNA aptamers that bind to the HA1 protein were isolated and shown to have different binding capacities. Among them, aptamer 10 had the strongest binding to the HA1 protein, and had an inhibitory effect on H5N1 influenza virus, as shown by the hemagglutinin and MTT assays. These results should aid the development of new drugs for the prevention and control of influenza virus infections.
迫切需要新的疗法来治疗由H5N1流感病毒突变体引起的大流行性流感。适体是治疗和预防流感病毒感染的一个有前景的候选物。在本研究中，通过指数富集的配体系统进化（SELEX）用于筛选靶向H5N1流感病毒重组HA1蛋白的DNA适体。经过11轮筛选，分离出与HA1蛋白结合的DNA适体，并显示出不同的结合能力。其中，适体10与HA1蛋白的结合最强，并且如血凝素和MTT试验所示，对H5N1流感病毒具有抑制作用。这些结果应有助于开发预防和控制流感病毒感染的新药。
