Gandia Carolina, Armiñan Ana, García-Verdugo Jose Manuel, Lledó Elisa, Ruiz Amparo, Miñana M Dolores, Sanchez-Torrijos Jorge, Payá Rafael, Mirabet Vicente, Carbonell-Uberos Francisco, Llop Mauro, Montero Jose Anastasio, Sepúlveda Pilar
Fundación Hospital General Universitario, Consorcio Hospital General Universitario de Valencia, Avenida Tres Cruces s/n, 46014 Valencia, Spain.
Stem Cells. 2008 Mar;26(3):638-45. doi: 10.1634/stemcells.2007-0484. Epub 2007 Dec 13.
Human dental pulp contains precursor cells termed dental pulp stem cells (DPSC) that show self-renewal and multilineage differentiation and also secrete multiple proangiogenic and antiapoptotic factors. To examine whether these cells could have therapeutic potential in the repair of myocardial infarction (MI), DPSC were infected with a retrovirus encoding the green fluorescent protein (GFP) and expanded ex vivo. Seven days after induction of myocardial infarction by coronary artery ligation, 1.5 x 10(6) GFP-DPSC were injected intramyocardially in nude rats. At 4 weeks, cell-treated animals showed an improvement in cardiac function, observed by percentage changes in anterior wall thickening left ventricular fractional area change, in parallel with a reduction in infarct size. No histologic evidence was seen of GFP+ endothelial cells, smooth muscle cells, or cardiac muscle cells within the infarct. However, angiogenesis was increased relative to control-treated animals. Taken together, these data suggest that DPSC could provide a novel alternative cell population for cardiac repair, at least in the setting of acute MI.
人牙髓含有被称为牙髓干细胞（DPSC）的前体细胞，这些细胞具有自我更新和多向分化能力，还能分泌多种促血管生成和抗凋亡因子。为了研究这些细胞在心肌梗死（MI）修复中是否具有治疗潜力，用编码绿色荧光蛋白（GFP）的逆转录病毒感染DPSC并在体外进行扩增。通过冠状动脉结扎诱导心肌梗死后7天，将1.5×10⁶个GFP-DPSC心肌内注射到裸鼠体内。4周时，通过观察前壁增厚百分比变化和左心室分数面积变化来评估心脏功能改善情况，发现细胞治疗组动物心脏功能有所改善，同时梗死面积减小。在梗死区内未发现GFP⁺内皮细胞、平滑肌细胞或心肌细胞的组织学证据。然而，与对照治疗动物相比，血管生成增加。综上所述，这些数据表明，至少在急性心肌梗死的情况下，DPSC可为心脏修复提供一种新的替代细胞群体。
