精氨酸血管加压催产素（AVT）和AVT相关肽是牛蛙神经中间叶中主要的醛固酮释放因子。

Arginine vasotocin (AVT) and AVT-related peptide are major aldosterone-releasing factors in the bullfrog neurointermediate lobe.

作者信息

Iwamuro S, Hayashi H, Yamashita M, Kikuyama S

机构信息

Department of Biology, School of Education, Waseda University, Tokyo, Japan.

出版信息

Gen Comp Endocrinol. 1991 Dec;84(3):412-8. doi: 10.1016/0016-6480(91)90089-o.

DOI:10.1016/0016-6480(91)90089-o

PMID:1808022

Abstract

Two major components which stimulate aldosterone release from Xenopus adrenocortical tissue were isolated from an acid-acetone extract of the neurointermediate lobes of the bullfrog (Rana catesbeiana) using C18 Sep-Pak cartridges, Sephadex G-50, and reverse-phase HPLC columns. One of the components was identified as arginine vasotocin (AVT) from its HPLC profile and amino acid sequence analysis. The other was an AVT-like decapeptide with an extra glycine residue at the C-terminus of nonamidated AVT, which was recently termed hydrin 2. The yields of these two peptides were almost the same. They also showed equipotent activity in stimulating water flux from the isolated urinary bladder of the toad (Bufo japonicus).

摘要

利用C18 Sep-Pak柱、Sephadex G-50和反相高效液相色谱柱，从牛蛙（Rana catesbeiana）神经中间叶的酸丙酮提取物中分离出两种刺激非洲爪蟾肾上腺皮质组织释放醛固酮的主要成分。其中一种成分通过高效液相色谱图谱和氨基酸序列分析被鉴定为精氨酸血管催产素（AVT）。另一种是在非酰胺化AVT的C末端带有一个额外甘氨酸残基的AVT样十肽，最近被称为hydrin 2。这两种肽的产量几乎相同。它们在刺激日本蟾蜍（Bufo japonicus）离体膀胱水通量方面也表现出同等效力。