Bond Chris
National Collection of Yeast Cultures, BBSRC Institute of Food Research, Colney, Norwich, UK.
Methods Mol Biol. 2007;368:109-17. doi: 10.1007/978-1-59745-362-2_7.
A method is described that allows a wide range of yeast species to be stored in liquid nitrogen while maintaining a high level of viability. Yeast cultures are sealed in commercially available polypropylene straws after having been mixed with a glycerol-based cryoprotectant. Once placed in a secondary cryotube the temperature of the sealed straws is reduced slowly to -30 degrees C in a methanol bath over a period of up to 3 h. The straws are then transferred directly to the liquid nitrogen and placed in a racking system for long-term storage.
