Kondrats'kyĭ A P, Sotkis H V, Boldyriev O I, Kondrats'ka K O, Liubanova O P, Dyskina Iu B, Hordiienko D V, Shuba Ia M
Fiziol Zh (1994). 2007;53(5):3-13.
For the first time by means of electrophysiology we demonstrate functional expression of cold/menthol sensitive TRPM8 channel in secretory epithelial cells plasma membrane from rat prostate. Under whole-cell patch-clamp technique rapid cooling from 33 to 21 degrees C of freshly isolated rat prostate epithelial cells elicited the significant outward rectifying membrane current with close to 0 mV reversal potential characteristic of TRPM8 activation. Extracellular application of chemical analog of cooling, menthol (100 microM) at 33 degrees C evoked similar current responses. Cold- and menthol-evoked currents could be detected only in elongated epithelial cells, which in prostate slices were labeled with anti-cytokeratin 8, suggesting that they belong to the apical secretory phenotype. No such current could be detected in round apparently basal epithelial cells. We conclude that in normal rat prostate functional expression of TRPM8 is restricted to plasma membrane of luminal apical epithelial cells.
我们首次通过电生理学方法证明，在大鼠前列腺分泌上皮细胞质膜中，冷/薄荷醇敏感的TRPM8通道存在功能性表达。在全细胞膜片钳技术下，将新鲜分离的大鼠前列腺上皮细胞从33℃快速冷却至21℃，引发了显著的外向整流膜电流，其反转电位接近0 mV，这是TRPM8激活的特征。在33℃时，细胞外应用冷却的化学类似物薄荷醇（100 microM）可诱发类似的电流反应。仅在细长的上皮细胞中可检测到冷和薄荷醇诱发的电流，在前列腺切片中，这些细胞用抗细胞角蛋白8标记，表明它们属于顶端分泌表型。在圆形的明显基底上皮细胞中未检测到这种电流。我们得出结论，在正常大鼠前列腺中，TRPM8的功能性表达仅限于管腔顶端上皮细胞的质膜。
