Morsczeck Christian, Korenkov Michael, Nagelschmidt Manfred, Feher Domonkos, Schierholz Jörg Michael
Stiftung Caesar, Bonn, Germany.
Prep Biochem Biotechnol. 2008;38(1):87-93. doi: 10.1080/10826060701774387.
Increasing complications in incisional hernia surgery call for novel treatments. A gene expression analysis of injured tissues displays important parameters for tissue regeneration. Until today, no reliable method has been described for a quantitative gene expression analysis of hernia tissues. In this work, a protocol is described for the isolation of DNA-free total RNA of incisional hernias for the first time. Moreover, real-time RT PCR assays for collagen type I and III and TGF-beta1 are demonstrated for relative gene expression analyses. Both methods enable relative gene expression analyses of hernia tissues for the first time.
切口疝手术中日益增多的并发症需要新的治疗方法。对损伤组织进行基因表达分析可显示组织再生的重要参数。迄今为止，尚未描述出一种用于疝组织定量基因表达分析的可靠方法。在这项工作中，首次描述了一种从切口疝中分离无DNA总RNA的方案。此外，还展示了针对I型和III型胶原蛋白以及转化生长因子β1的实时逆转录聚合酶链反应（RT PCR）检测，用于相对基因表达分析。这两种方法首次实现了对疝组织的相对基因表达分析。
