Imanari T, Oguma T, Akiyama H, Toyoda H, Koshiishi I
Faculty of Pharmaceutical Sciences, Chiba University, Japan.
J Pharmacobiodyn. 1991 Nov;14(11):631-5. doi: 10.1248/bpb1978.14.631.
Platelet proteo-chondroitin sulfates localize on the surface and in the granules. Western blotting method is able to selectively detect core-proteins prepared from proteo-chondroitin sulfates by chondroitinase ABC digestion. A very highly sensitive high-performance liquid chromatographic method was established for the determination of chondroitin sulfates as their unsaturated disaccharides. These methods were applied to rabbit platelet proteo-chondroitin sulfates. It was found that both rabbit platelet surface proteo-chondroitin sulfate and granule proteo-chondroitin sulfate consist of a predominantly homogeneous chondroitin 4-sulfate and a single core-protein having a molecular weight of approximately 26 kDa.
血小板蛋白聚糖硫酸软骨素定位于表面和颗粒中。蛋白质印迹法能够通过软骨素酶ABC消化选择性地检测从蛋白聚糖硫酸软骨素制备的核心蛋白。建立了一种非常灵敏的高效液相色谱法，用于测定硫酸软骨素的不饱和二糖。这些方法应用于兔血小板蛋白聚糖硫酸软骨素。结果发现，兔血小板表面蛋白聚糖硫酸软骨素和颗粒蛋白聚糖硫酸软骨素均主要由均一的硫酸软骨素4-硫酸酯和一种分子量约为26 kDa的单一核心蛋白组成。
