作为进食、循环脂质和储存脂肪调节剂的肥胖抑制素片段。
Fragments of obestatin as modulators of feed intake, circulating lipids, and stored fat.
作者信息
Nagaraj ShreeRanga, Peddha Muthukumar S, Manjappara Uma V
机构信息
Department of Protein Chemistry and Technology, Central Food Technological Research Institute, Cheluvamba Mansion, Mysore 570020, Karnataka, India.
出版信息
Biochem Biophys Res Commun. 2008 Feb 15;366(3):731-7. doi: 10.1016/j.bbrc.2007.12.036. Epub 2007 Dec 17.
Obestatin, shown to reduce feed intake and gain in body weight in rodents, is a very attractive candidate to be used against obesity. In this study, we aimed to investigate the relationship between the primary structure and activity of obestatin. Also of interest to us is a peptide of minimal length that closely mimics obestatin. Towards the same, we synthesized rodent obestatin and three overlapping fragments spanning residues 1-13, 6-18, and 11-23 of obestatin. These peptides subsequent to purification and characterization were tested upon adult male mice for their ability to reduce feed intake and gain in body weight. The N-terminal peptide (residues 1-13) mimicked obestatin the closest. The middle fragment (residues 6-18) significantly reduced epididymal fat without much altering feed intake or body weight.
肥胖抑制素已被证明可减少啮齿动物的采食量并降低体重增加，是用于对抗肥胖的极具吸引力的候选物。在本研究中，我们旨在研究肥胖抑制素的一级结构与活性之间的关系。我们还对一种长度最短且紧密模拟肥胖抑制素的肽感兴趣。为此，我们合成了啮齿动物肥胖抑制素以及跨越肥胖抑制素第1 - 13、6 - 18和11 - 23位残基的三个重叠片段。这些肽在纯化和表征后，对成年雄性小鼠进行了测试，以检测它们减少采食量和体重增加的能力。N端肽（第1 - 13位残基）最接近地模拟了肥胖抑制素。中间片段（第6 - 18位残基）显著减少了附睾脂肪，而对采食量或体重没有太大影响。
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