Lu Xianbo, Zhou Jianhua, Lu Wu, Liu Qing, Li Jinghong
Department of Chemistry, Key Laboratory of Bioorganic Phosphorus Chemistry & Chemical Biology, Tsinghua University, Beijing 100084, China.
Biosens Bioelectron. 2008 Mar 14;23(8):1236-43. doi: 10.1016/j.bios.2007.11.006. Epub 2007 Nov 17.
Carbon nanofibers (CNFs), with typical diameters of approximately 80 nm and lengths of the order of micrometers, are extremely attractive in bioanalytical area as they can combine properties of high surface area, non-toxicity, acceptable biocompatibility, ease of fabrication, chemical and electrochemical stability, good electrical conductivity. In this work, CNF-based composites were successfully used as an immobilization matrix for the construction of a reagentless mediator-free hemoglobin-based H2O2 biosensor. The results revealed that hemoglobin retained its essential secondary structure in the CNF-based composite film. With the advantages of organic-inorganic hybrid materials, dramatically facilitated direct electron transfer of hemoglobin and good bioelectrocatalytic activity towards H2O2 were demonstrated. The biosensor displayed good performance along with good long-term stability. The CNF-based composites were proved to be a promising biosensing platform for the construction of mediator-free biosensors, and may find wide potential applications in biosensors, biocatalysis, bioelectronics and biofuel cell.
碳纳米纤维（CNFs）的典型直径约为80纳米，长度在微米量级，在生物分析领域极具吸引力，因为它们可以兼具高比表面积、无毒、可接受的生物相容性、易于制备、化学和电化学稳定性以及良好的导电性等特性。在这项工作中，基于碳纳米纤维的复合材料成功用作固定基质，用于构建无试剂、无媒介体的基于血红蛋白的H2O2生物传感器。结果表明，血红蛋白在基于碳纳米纤维的复合膜中保留了其基本二级结构。凭借有机-无机杂化材料的优势，显著促进了血红蛋白的直接电子转移，并展示出对H2O2良好的生物电催化活性。该生物传感器表现出良好的性能以及良好的长期稳定性。基于碳纳米纤维的复合材料被证明是构建无媒介体生物传感器的一个有前景的生物传感平台，并且可能在生物传感器、生物催化、生物电子学和生物燃料电池中找到广泛的潜在应用。
