Nishida Kinya, Horiuchi Masataka, Noda Nobuo N, Takahasi Kiyohiro, Iwasaki Norimasa, Minami Akio, Inagaki Fuyuhiko
Department of Structural Biology, Graduate School of Pharmaceutical Science, Hokkaido University, N12, W6, Kita-ku, Sapporo 060-0812, Japan.
Acta Crystallogr Sect F Struct Biol Cryst Commun. 2007 Dec 1;63(Pt 12):1061-3. doi: 10.1107/S1744309107057466. Epub 2007 Nov 30.
The Tob/BTG family is a group of antiproliferative proteins that contain two highly homologous regions named Box A and Box B. These proteins all associate with CCR4-associated factor 1 (Caf1), which belongs to the ribonuclease D family of deadenylases. The antiproliferative region of human Tob (residues 1-138) and intact hCaf1 were co-expressed in Escherichia coli, purified and successfully cocrystallized. The crystal belongs to the tetragonal space group I422, with unit-cell parameters a = b = 150.9, c = 113.9 A, and is estimated to contain one heterodimer per asymmetric unit. The crystal diffracted to around 2.6 A resolution.
Tob/BTG家族是一组抗增殖蛋白，包含两个高度同源的区域，分别称为A盒和B盒。这些蛋白都与CCR4相关因子1（Caf1）相关联，Caf1属于去腺苷酸化酶的核糖核酸酶D家族。人Tob的抗增殖区域（第1至138位氨基酸残基）和完整的hCaf1在大肠杆菌中共同表达、纯化并成功进行了共结晶。该晶体属于四方晶系空间群I422，晶胞参数a = b = 150.9，c = 113.9 Å，估计每个不对称单元包含一个异源二聚体。该晶体的衍射分辨率约为2.6 Å。
