Kashyap Luv, Tabish Mohammad, Ganesh Ganesh, Dubey Deepti
Bioinformation. 2007 May 30;2(1):17-21. doi: 10.6026/97320630002017.
C. elegans C46H11.4 gene encodes a Let-23 fertility effector/regulator protein of the EGF-receptor class of the tyrosine kinase family. Alternative splicing is a major mechanism of generating protein diversity in higher eukaryotes. C. elegans genome sequencing consortium has reported three alternatively spliced transcripts of C46H11.4 gene which encodes for three hypothetical proteins namely, C46H11.4a, C46H11.4b and C46H11.4c. Using a combination of various bioinformatics tools like gene or exon finding programmes, blast searches, alignment tools etc followed by experimental validation, we report the presence of three more alternatively spliced transcripts which encode for novel hypothetical proteins C46H11.4d, C46H11.4e and C46H11.4f. These isoforms arise as a result of alternative splicing in the pre-mRNA encoded by gene C46H11.4. These novel un-reported spliced variants not only point towards the extent of alternative splicing in C. elegans genes but also hint towards the complex nature of alternative splicing.
秀丽隐杆线虫的C46H11.4基因编码一种酪氨酸激酶家族表皮生长因子受体类的Let-23生育效应器/调节蛋白。可变剪接是高等真核生物中产生蛋白质多样性的主要机制。秀丽隐杆线虫基因组测序联盟报告了C46H11.4基因的三种可变剪接转录本，它们编码三种假定蛋白，即C46H11.4a、C46H11.4b和C46H11.4c。通过结合使用各种生物信息学工具，如基因或外显子查找程序、比对搜索、比对工具等，随后进行实验验证，我们报告了另外三种可变剪接转录本的存在，它们编码新的假定蛋白C46H11.4d、C46H11.4e和C46H11.4f。这些异构体是由C46H11.4基因编码的前体mRNA中的可变剪接产生的。这些新的未报道的剪接变体不仅表明了秀丽隐杆线虫基因中可变剪接的程度，也暗示了可变剪接的复杂性质。
