Lee Karen Ying, Liu Yi-Hsiang, Ho Chin-Chin, Pei Ren-Jeng, Yeh Kun-Tu, Cheng Chiung-Chi, Lai Yih-Shyong
Center for Research and Development, Chungtai Institute of Health Sciences and Technology, Taichung, Taiwan.
J Med. 2004;35(1-6):141-9.
Tumor cells are morphologically different from normal cells. In this situation, we proposed that plectin, one of the intermediate filament associated proteins, might play some special roles in the tumorigenesis. Plectin exhibits a wide distribution spectrum among various tissues; however, it is scarcely investigated in tumor tissues including colorectal adenocarcinoma. In this study, we searched the plectin expression in 25 cases of colorectal adenocarcinoma and 10 cases of tubular adenoma with focal adenocarcinoma by immunohistochemical method. The results reveal that plectin is up-regulated in colorectal adenocarcinoma as well as in bizarre glands and locally invasive tumor nests in tubular adenoma, compared with normal colorectal mucosa. Over-expression of plectin in locally invasive tumor nests might help us in diagnosis of distinguish microinvasive foci from normal glands. The bizarre glands in adenoma might be precancerous lesions since the expression of plectin in them was identical to that of adenocarcinoma but in contrast to that of normal glands. The overexpression of plectin might affect the organization of cytoskeleton, which might further cause tumorigenesis and morphological change of colorectal epithelial cells.
肿瘤细胞在形态上与正常细胞不同。在这种情况下，我们提出，中间丝相关蛋白之一的网蛋白可能在肿瘤发生过程中发挥一些特殊作用。网蛋白在各种组织中分布广泛；然而，在包括结直肠腺癌在内的肿瘤组织中对其研究甚少。在本研究中，我们采用免疫组织化学方法检测了25例结直肠腺癌和10例伴有局灶腺癌的管状腺瘤中网蛋白的表达情况。结果显示，与正常结直肠黏膜相比，网蛋白在结直肠腺癌以及管状腺瘤的怪异腺体和局部浸润性肿瘤巢中表达上调。网蛋白在局部浸润性肿瘤巢中的过表达可能有助于我们将微浸润灶与正常腺体区分开来进行诊断。腺瘤中的怪异腺体可能是癌前病变，因为其中网蛋白的表达与腺癌相同，但与正常腺体相反。网蛋白的过表达可能会影响细胞骨架的组织，进而可能导致结直肠上皮细胞发生肿瘤形成和形态改变。
