Zuperku Edward J, Brandes Ivo F, Stucke Astrid G, Sanchez Antonio, Hopp Francis A, Stuth Eckehard A
Medical College of Wisconsin, Anesthesiology Department, USA.
Adv Exp Med Biol. 2008;605:279-84. doi: 10.1007/978-0-387-73693-8_49.
Multibarrel micropipettes were used to simultaneously record unit activity and apply antagonists on individual inspiratory hypoglossal motoneurons (IHMNs) to determine the endogenous activation levels of NMDA, non-NMDA, GABA(A) and serotonin receptors responsible for the IHMN spontaneous discharge patterns in decerebrate dogs. IHMN activity is highly dependent on glutamatergic phasic and tonic drives, which are differentially mediated by the receptor subtypes. Endogenous serotonin significantly amplifies IHMN activity, while GABAergic gain modulation acts to attenuate activity. Thus, alterations in the neurotransmission of any of these systems could markedly alter neuronal output to target muscles.
使用多管微量移液器同时记录单位活动，并对单个吸气舌下运动神经元（IHMNs）施加拮抗剂，以确定负责去脑犬IHMN自发放电模式的NMDA、非NMDA、GABA(A)和血清素受体的内源性激活水平。IHMN活动高度依赖于谷氨酸能的相位性和紧张性驱动，这些驱动由受体亚型差异介导。内源性血清素显著增强IHMN活动，而GABA能增益调制则起到减弱活动的作用。因此，这些系统中任何一个的神经传递改变都可能显著改变对靶肌肉的神经元输出。
