Masaki Shunpei, Yamada Tetsuya, Hirasawa Tadashi, Todaka Daisuke, Kanekatsu Motoki
Department of Plant Production United Graduate School of Agricultural Science, Tokyo University of Agriculture and Technology, Fuchu, 183-8509, Tokyo, Japan.
Biotechnol Lett. 2008 May;30(5):955-60. doi: 10.1007/s10529-007-9619-8. Epub 2007 Dec 18.
In proteomic analysis, one of the major limitations is the detection of low-abundance proteins. To detect low-abundance RNA-binding proteins in mature dry seeds of rice, fractionation by single stranded DNA (ssDNA) affinity column chromatography was carried out before analysis by two-dimensional gel electrophoresis (2-DE). Proteomic analysis of the ssDNA-binding fraction revealed the existence of three types of RNA-binding proteins, including a K homology (KH) domain containing protein, a putative RNA-binding protein and a glycine-rich RNA-binding protein, in mature seeds. In addition, decreases in the putative RNA-binding protein and glycine-rich RNA-binding protein after absorbing water in seeds appear to be associated with seed germination.
在蛋白质组学分析中，一个主要限制是低丰度蛋白质的检测。为了检测水稻成熟干燥种子中的低丰度RNA结合蛋白，在通过二维凝胶电泳（2-DE）分析之前，进行了单链DNA（ssDNA）亲和柱色谱分离。对ssDNA结合部分的蛋白质组学分析揭示了成熟种子中存在三种类型的RNA结合蛋白，包括一种含K同源（KH）结构域的蛋白质、一种假定的RNA结合蛋白和一种富含甘氨酸的RNA结合蛋白。此外，种子吸水后假定的RNA结合蛋白和富含甘氨酸的RNA结合蛋白的减少似乎与种子萌发有关。
